棘球蚴感染的检测分析

摘要

目的 探讨动物宿主棘球蚴感染的检测方法,为绦虫感染的治疗与预防提供参考.方法 选择泡状棘球蚴病羊血清和囊型棘球蚴病羊血清各100份,建立AgB原核表达体系,采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分析及酶联免疫吸附测定(ELISA)进行血清学诊断评价.结果 联合表达抗原AgBs对囊型棘球蚴病羊血清的敏感性和特异性均为90.0%,明显高于AgB1对囊型棘球蚴病羊血清的敏感性和特异性(分别为70.0%、56.0%)(P<0.05).结论 联合表达抗原AgBs对囊型棘球蚴病的诊断价值优于AgB1.%Objective To explore the detection methods of echinococcus infection in animal host and provide a reference for prevention and treatment of tapeworm infection. Methods Serum samples of goats with cystic echinococcosis and with alveolar echino-coccosis (each of 100) were collected. AgB prokaryotic expression system was established. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGK) was employed to analyze AgB expression and enzyme-linked immunosorbent assay(KLISA) was a-dopted to evaluate the serological diagnosis. Results Sensitivity and Specificity of co-expression antigen AgBs to sera of goats with cystic echinococcosis were both 90. 0% , which were higher than those of AgB1(70. 0% and 56. 0% , respectively) (P<0. 05). Conclusion The diagnostic value of co-expression antigen AgBs in cystic echinococcosis is prior to that of antigen AgB1.