'immunoassay method for detecting an analyte, reagent kit for detecting an analyte by an immunoassay, and use of a marker-specific binding partner'

摘要

The invention relates to an immunoassay method for detecting an analyte, such as an antigen or an antibody in an isolated sample suspected of containing said analyte, by incubating said sample with a plurality of binding partners, one of which having a detectable marker, wherein a marker-specific binding partner is added that does not have a marker but binds to said detectable marker. The method is applicable to a wide variety of analytes and has proven to be particularly useful for IgG and IgM class antibodies present in samples due to pathogen infections. The invention further relates to a reagent kit useful for said method comprising at least two analyte specific binding partners, one of which having a detectable marker and a marker-specific binding partner that binds to said detectable marker. , but has no detectable marker. In addition, the invention encompasses the use of a marker-specific binding partner that lacks a detectable marker in an in vitro diagnostic test to eliminate interference caused by anti-marker antibodies present in a sample.