目的 通过建立大鼠在限制性应激条件下实验性牙周炎动物模型,探讨限制性应激在实验性牙周炎发展过程中的作用.方法 48只雄性SD大鼠用0.012 mm不锈钢丝线结扎左上颌第一磨牙后,随机分为两组,一组为应激组(给予限制性应激),另一组为非应激组(单纯结扎),结扎后1、2、3、4周后检测牙龈指数.每组大鼠分别在施加应激后第2、3、4、5周随机各取6只处死,应激指标促肾上腺皮质激素(ACTH)、皮质酮激素(CORT)在血清中的含量,以及牙槽骨丧失程度(AL).结果 大鼠结扎后的左上颌第一磨牙牙龈指数均高于未结扎的右上颌第一磨牙,差异有统计学意义(P<0.01);应激组ACTH和CORT含量均高于非应激组(P<0.01);应激组结扎区AL明显高于非应激组,差异有统计学意义(P<0.01);应激组前4周AL与时间成正比,差异有统计学意义(P<0.05).结论 应激可以加重牙槽骨的吸收,并且随着应激时间的增加,牙槽骨吸收的程度也会有所增加.%Objective To investigate the effect of restraint stress on the development and progression of experimental periodon titis. Methods Forty eight male SD rats were selected with stainless steel wire (0. 012mm) ligating the left maxillary first molar. They were randomly divided into two groups: stress group (the rats were treated with restraint stress and ligation) and control group (the rats were treated with simple ligation). 6 rats selected randomly from both groups were executed at the end of the 2nd, 3rd, 4th and 5th week respectively after applied stress. The related gingival index(GI) ,the levels of ACTH and CORT in serum,and the degree of AL were tested. Results The GI in rats' left maxillary first molar ligating with the stainless steel wire were signifi cant higher than the right maxillary first molar without ligation (P<0. 01). The contents of ACTH and CORT,and the degree of AL in stress group were significantly increased (P<0. 01) compared with control group. And with extension of the stress time,the AL level was positively relative to the time in the first four weeks (P<0. 05). Conclusion Restraint stress can aggravating rat pe riodontitis progress,and it can increase alveolar bone resorption with prolongationof time.
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