短柱八角化学成分及其抗炎活性的研究

摘要

AIM To study the chemical constituents from Illicium brevistylum A.C.Smith and to evaluate their anti-inflammatory activities.METHODS The n-BuOH fraction of 80％ ethanol extract from I.brevistylum was isolated and purified by silica,ODS and Sephadex LH-20,the structures of obtained compounds were identified by spectral data.Then their anti-inflammatory activities were screened.RESULTS Nine compounds were isolated and identified as (7S,8R)-3,3',5-trimethoxy-4',7-epoxy-8,5'-neolignan-4,9,9'-triol (1),methylabieta-8,11,13,15-tetraen-18-oate (2),majusanin A (3),pubeside C (4),(+)-lyoniresinol-3a-O-α-L-rhamnopyranoside (5),junipercomnoside D (6),lyoniside (7),nudiposide (8),rhyncoside A (9).The inhibition rates of compounds 2,6,9 on NF-κB were 47.81％,37.33％,33.37％,respectively.CONCLUSION Compounds 1,5,7-9 are isolated from genus Illicium for the first time,and compounds 2,6,9 exhibit good anti-inflammatory activities.%目的 研究短柱八角Illicium brevistylum A.C.Smith的化学成分,并评价其抗炎活性.方法 短柱八角80％乙醇提取物的正丁醇部位采用硅胶、ODS、Sephadex LH-20进行分离纯化,经波谱数据鉴定所得化合物的结构,再对其抗炎活性进行筛选.结果 从中分离出9个化合物,分别鉴定为(7S,8R)-3,3',5-trimethoxy-4',7-epoxy-8,5'-neolignan-4,9,9'-triol(1)、methylabieta-8,11,13,15-tetraen-18-oate (2)、majusanin A(3)、豨莶丙苷(4)、(+)-lyoniresinol-3 a-O-α-L-rhamnopyranoside (5)、junipercomnoside D(6)、南烛木糖苷(7)、nudiposide(8)、尖瓣海莲苷A(9).化合物2、6、9对NF-κB的抑制率分别为47.81％、37.33％、33.37％.结论 化合物1、5、7～9为首次从八角属植物中分离得到,化合物2、6、9具有较好的抗炎活性.