4个栽培年限丹参颜色与9种活性成分含量的相关性

摘要

目的 研究4个栽培年限(1、2、3、5年)丹参Salvia miltiorrhiza Bge.颜色与9种活性成分含有量的相关性.方法 精密色差仪测定色度值(L*、a*、b*),HPLC法测定水溶性成分(紫草酸、丹酚酸B、迷迭香酸、原儿茶醛酸、丹参素钠)和脂溶性成分(二氢丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ)含有量,通过Pearson相关性分析考察颜色与各成分含有量的相关性.结果 2年生丹参的L *、b *值最高,3年生者a*值最高,5年生者各色度值均最低。各成分含有量均在3年生者中达到最高.紫草酸、丹参素钠与各色度值均无显著相关性,丹酚酸B、迷迭香酸、丹参酮ⅡA与其均呈显著正相关,原儿茶醛酸与其均呈显著负相关.结论 栽培年限为3年的丹参质量较高.%AIM To study the correlations between colors and contents of nine active constituents of Salvia miltiorrhiza Bge.with four growth years (one,two,three and five years).METHODS The chromatic values (L*,a* and b*) were determined by precise color difference meter.HPLC was applied to determining the contents of water-soluble constituents (lithospermic acid,salvianolic acid B,rosmarinic acid,protocatechuic aldehyde acid and salvianic acid A sodium) and liposoluble-soluble constituents (dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ A).Then the correlations between colors and contents of various constituents were investigated by Pearson correlation analysis.RESULTS L* and b * values of two growth-year S.miltiorrhiza,together with a* value of three growth-year S.miltiorrhiza,were the highest,but various chromatic values of five growth-year S.miltiorrhiza were all the lowest.The contents of various constituents in three growth-year S.miltiorrhiza reached the highest.Lithospermic acid and salvianic acid A sodium showed no significant correlations with various chromatic values,salvianolic B,rosmarinic acid and tanshinone Ⅱ A exhibited significantly positive correlations with them,and protocatechuic aldehyde had significantly negative correlations with them.CONCLUSION The quality of three growth-year S.miltiorrhiza is relatively high.