丹参根部颜色及其与活性成分含量的相关性研究

摘要

采用 HPLC 法测定丹参根部水溶性（迷迭香酸、紫草酸、丹酚酸 B）和脂溶性（二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA）活性成分含量，并用色差仪测根部颜色，探讨丹参根部颜色与其活性成分含量间的相关性，为制定丹参药材等级标准提供依据。结果表明：不同颜色级别丹参表面色度值存在明显差异，可据此对其进行等级划分；多数活性成分含量存在显著差异；二氢丹参酮Ⅰ和紫草酸与色度值 L*、a*、b*相关性不显著（P ＞0．05），其他5种成分与色度值 L*均呈负相关，与 a*、b*均呈正相关，其中迷迭香酸、丹酚酸 B 及丹参酮Ⅰ与色度 L*值呈极显著负相关（P ＜0．01），隐丹参酮、丹参酮ⅡA 与 a*值和 b*值呈现极显著正相关（P ＜0．01）。由此可见，丹参根部越红活性成分含量越高，这为合理评价丹参药材质量、进行丹参药材分级提供了理论依据。%The HPLC method was applied to determine the contents of water -soluble ingredients (ros-marinic acid,lithospermic acid,Salvianolic B)and fat -soluble ingredients (DihydrotanshinoneⅠ,crypto-tanshinone,tanshinoneⅠ and tanshinone ⅡA).The color of RADIX SALVIAE MILTIORRHIZAE was meas-ured by colorimeter.The correlation between them was analyzed to provide references for setting grade stand-ard of RADIX SALVIAE MILTIORRHIZAE.The results showed that the chromatic value of RADIX SALVIAE MILTIORRHIZAE surface was different between different grades,so grading could conduct based on this;and the content of each ingredient was obviously different between different colour grades.Except Dihydrotanshi-noneⅠand lithospermic acid had no significant correlations with the chromatic value L*,a* and b* (P >0.05),the other ingredients all showed negative correlations with L* but positive correlations with a* and b*.Among them,rosmarinic acid,Salvianolic B and tanshinoneⅠ showed very significantly negative correla-tions with L*(P <0.01),while cryptotanshinone and tanshinone ⅡA showed very significantly positive corre-lations with a* and b*(P <0.01).Thus,the redder the RADIX SALVIAE MILTIORRHIZAE was,the higher the contents of active ingredients were,which could provide theoretical references for reasonable quality evalu-ation and grading of RADIX SALVIAE MILTIORRHIZAE.