目的：探讨OK-432肿瘤细胞疫苗对DBA/2小鼠CD4+CD25+Treg细胞免疫效应。方法本研究利用7,12-二甲基苯蒽（DMBA）/巴豆油二阶段诱癌法建立DBA/2小鼠皮肤癌荷瘤模型，将荷瘤小鼠随机分为3组，分别为OK-432疫苗组，OK-432组和空白对照组，各组小鼠于左侧腹腔分别注射浓度为5×105个/100μl的OK-432肿瘤细胞疫苗100μl，0.7 KE/100μl的OK-432100μl磷酸盐缓冲液（PBS）液100μl，每周注射1次，连续注射3周，比较和分析不同实验组皮肤癌荷瘤小鼠的免疫应答。结果药物末次干预后第1、3、7、14天, OK-432疫苗组和空白对照组相比CD4+CD25+Treg细胞明显降低，差异均具有统计学意义（P<0.05）。结论本实验证实OK-432肿瘤疫苗能减少CD4+CD25+Treg细胞的含量，可以降低机体免疫抑制功能。%Objective To investigate the immune effects of OK-432 tumor cell vaccine on the CD4+CD25+Treg cells in DBA/2 mice. Methods The 7, 12-DMBA/croton oil two-stage method of carcinogenesis was used to build DBA/2 mice tumor-bearing model of skin cancer. And the tumor-bearing mice were randomly divided into three groups, which were the OK-432 vaccine group, OK-432 group and control group. The mice in each group were inject-ed with 100μl OK-432 tumor cell vaccine (5×105/100μl concentration), OK-432 (0.7 KE/100μl) and phosphate buffer saline (PBS) respectively on the left abdominal cavity once a week for continuous three weeks. The immune re-sponses of skin cancer tumor-bearing mice in different experimental groups were compared and analyzed. Results At days 1, 3, 7, 14 after the last drug intervention, the CD4+CD25+Treg cells were significantly reduced in the OK-432 vaccine group than that in the control group, with statistically significant differences ( P<0.05). Conclusion Our ex-periment confirmed that the OK-432 tumor vaccine could reduce the content of CD4+CD25+Treg cells and body im-mune suppression function.
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