中国被毛孢菌丝体提取物抗肾损伤作用体内外实验研究

摘要

目的 探讨中国被毛孢菌丝体提取物(HSW)抗肾损伤的药理作用.方法 采用体外培养的人肾小管上皮细胞株(HK2)模型,观察HSW对马兜铃酸(AA)造成的HK2细胞损伤的逆转作用,及对于肾衰竭相关功能指标的影响.并在庆大霉素致大鼠急性肾衰竭(ARF)模型上验证HSW的作用.结果 HSW可以逆转AA对HK2细胞所造成的损伤,有效地抑制AA 引起的HK2 细胞TGFβ1、PAI-1 mRNA表达的上调,并可保护庆大霉素造成的大鼠急性肾损伤.结论 HSW对肾损伤的体内外模型有较好的药理作用.%Aim To investigate the effect of Hirsutella sinensis extract ( HSW ) on kidney injury. Methods The inversion effect of HSW on the renal tubular epithelial cell ( HK2 ) injury caused by aristolochic acid ( AA ) was observed, its influence on functional parameters of renal failure was evaluated and the effect of HSW on acute renal failure ( ARF ) of rats induced by gentamicin was validated. Results HSW deterioratedHK2 cell damage caused by AA, inhibited the upregu-lation of the expression of TGFPj and PAI-1 mRNA, and protected rats from ARF caused by gentamicin. Conclusion HSW had a remarkable action in vitro and in vivo on model of kidney injury.