Monoclonal Anti-Idiotype Induces Protection Against the Cytotoxicity of the Trichothecene Mycotoxin T-2. (Reannouncement with New Availability Information).

摘要

The trichotecene mycotoxins, a group of chemically related fungal metabolities, represents a major source of contamination of agricultural products a major source of contamination of agricultural products. They are non-proteinaceous compounds of approximate molecular mass of (300) to (500) kDa, that are characterized by a basic =tetracyclic= =sesquiterpene= structure with a (12), (13)-epoxide group and a (9), (10)-=oleginic= bond. The range of biologic activities exhibited by the trichotecene mycotoxins include antifungal, antibacterial, and antiviral effects. Some are phytotoxic whereas all show some degree of animal toxicity. The trichotecene mycotoxin T-2 has been shown to mediate potent inhibition of protein synthesis. The site of this inhibition appears to reside at the initiation step on polyribosomes and the elongation and termination step in protein synthesis. Presumably, T-2 mycotoxin exerts its cytotoxicity by binding to the ribosomal subunit resulting in the inhibition of peptidyl transferase. Because of the chemical nature and structure of the mycotoxin T-2, and its potent toxicity, conventional approaches to developing vaccines agiainst T-2 such as synthetic peptide and recombinant technologies, or immunization with attenuated forms of native Ag may not be practical. Indeed conjugation of toxins such as T-2 to protein carriers has been shown to result in unstable complexes with potential release of free toxin in its active form. Thus, it appears that a reasonable approach to vaccine development against potent biologic toxins such as T-2 may be the anti-id-based approach.