黄芪有效成分对缺氧缺糖/复氧复糖大鼠海马神经元cyt-c、CcO表达的影响

摘要

目的 观察黄芪有效成分对缺氧缺糖/复氧复糖大鼠海马神经元cyt-c及CcO表达的影响,探讨黄芪有效成分防治海马神经元凋亡可能的作用机制.方法 取培养8 d的大鼠海马神经元,进行缺氧缺糖0.5 h,再分别复氧复糖0、0.5、2、6、24、72和120 h.实验分4组:正常对照组、模型组(缺氧缺糖/复氧复糖组)、黄芪注射液溶剂对照组和黄芪注射液组.分别采用细胞免疫化学法、Western blot法检测海马神经元cyt-c蛋白的表达、RT-PCR法检测CcO mRNA的表达.结果 模型组在各个时间点cyt-c蛋白和CcO mRNA表达均较正常对照组增加(P0.05).结论 黄芪注射液可抑制缺氧缺糖/复氧复糖大鼠海马神经元cyt-c、CcO的表达,从而抑制缺氧缺糖/复氧复糖后大鼠海马神经元的凋亡.%Aim To investigate the effect of astragalus injection on the expression of cyt-c and CcO after hypoxia /hypoglycemia and reoxygenation in hippocampal neurons of rats.Methods The hippocampal neurons cultured for eight days were divided into 4 groups : normal control group , model group( hypoxia/hypoglycemia and reoxygenation group ), original astragalus injection group , astragalus injection group.Model group , astragalus injection group and original astragalus injection group were treated with hypoglycemia and reoxygenation after deprived of oxygen and glucose for 0.5 h.Methods of immunocytochemistry, Western blot and RT-PCR were used respectively to measure the expression of cyt-c and CcO after hypoxia /hypoglycemia and reoxygenation 0.0.5,2.6,24.72 h and 120 h.Results Compared to normal control group , the expression of cyt-c protein and CcO mRNA at each time point in model group increased obviously( P＜0.05 ).Compared to model group, the expression of cyt-c protein and CcO mRNA at each time point in astragalus injection group decreased obviously( P ＜ 0.05 ), however, the expression of cyt-c protein and CcO mRNA in original astragalus injection group had no obvious change ( P ＞0.05 ).Conclusion Effective compositions of astragalus injection inhibits the expression of cyt-c and CcO after hypoxia/hypoglycemia and reoxygenation , accordingly inhibits hippocampal neuronal apoptosis.