目的 观察黄芪注射液对缺氧缺糖/复氧复糖大鼠海马神经元bcl-2、bax及cyt-c蛋白表达的影响,探讨黄芪注射液防治海马神经元凋亡的作用机制.方法 取原代培养8 d的大鼠海马神经元,随机分为4组:正常对照组、模型组(缺氧缺糖/复氧复糖组)、黄芪注射液溶剂对照组和黄芪注射液组.除正常对照组外,各组均进行缺糖缺氧0.5 h,再分别于复氧复糖后0、0.5、2、6、24、72和120 h采用细胞免疫化学法检测海马神经元bcl-2、bax、cyt-c蛋白的表达.结果 与正常对照组比,模型组bcl-2、bax、cyt-c蛋白表达明显升高,而bcl-2/bax比值下调(P＜0.05).与模型组相比,黄芪注射液组bcl-2蛋白表达及bcl-2/bax比值升高,cyt-c、bax蛋白表达明显降低(P＜0.05);而黄芪注射液溶剂对照组bcl-2、bax、cyt-c蛋白表达及bcl-2/bax比值则无显著变化(P＞0.05).结论 黄芪注射液可提高缺氧缺糖/复氧复糖大鼠海马神经元bcl-2蛋白表达及bcl-2/bax比值,抑制bax、cyt-c蛋白表达,从而抑制缺氧缺糖/复氧复糖引起的海马神经元凋亡.%Objective To investigate the effect of astragalus injection on the expression of bcl-2, bax,cyt-c protein after hypoxia/hypoglycemia and reoxygenation in hippocampal neurons of rats.Methods The hippocampal neurons cultured for 8 d were divided into normal control, model( hypoxia/hypoglycemia and reoxygenation),original astragalus injection and astragalus injection groups.Model group, astragalus injection group and original astragalus injection group were treated with hypoglycemia and reoxygenation after deprived of oxygen and glucose for 30 min.Method of immunocytochemistry was used respectively to measure the expression of bcl-2, bax, cyt-c protein after hypoxia / hypoglycemia and reoxygenation 0,0.5,2,6,24,72 and 120 h.Results Compared to normal control group,the expression of bcl-2 ,bax,cytc protein were increased obviously,the ratio of bcl-2/bax was decreased at each time point in model group( P ＜0.05).Compared to model group,the expression of bax,eyt-c protein were decreased obviously,bcl-2 protein and the ratio of bcl-2/bax were increased obviously at each time point in astragalus injection group( P ＜ 0.05 ), however the expression of bcl-2, bax, cyt-c protein in original astragalus injection group had no obvious change ( P ＞ 0.05 ).Conclusions Astragalus injection inhibits the expression of bax, cyt-c and increases bcl-2 and bcl-2/bax after hypoxia/hypoglycemia and reoxygenation, accordingly inhibits hippocampal neuronal apoptosis.
展开▼
