Aim To invesLigaLe vvheLher emodin induces autophagy in Lhe nasopharyngeal carcinoma CME-1 colls aftcr radiaLion combined with emodin and explore the relationship between the radiotherapy sensi-tization and autophagy-related gene mRMA expression. Methods Masopharyngeal carcinoma CME-1 cells were divided into control group, emodin alone group, radiation alone group and radiaLion combined with emodin group. After different treatmenLs, the ultrastructur-al changes of autophagosome were observed by trans-mission electron microscope( TEM ). The mRMA expressions of ULK.1 , GABARAPL1 , Beclinl and Bcl-2 were detected by Lhe Real-time PCR. Results Autophagosome or autophagolysosome was observed in all groups except in the control cells. Compared with the control group, the resuks of Real-time PCR showed that ULK1 mRMA expression in all the experimental groups increased( P <0. 05 ). The radiotherapy sensiti-zation group showed the highest( P <0. 01 ). However, Beclinl and Bcl-2 mRMA expression were down-regu-lated. GABARAPL1 expression had no remarkable changes among the experimental groups, exeept in radiotherapy sensitizaLion group where it was up-regulated ( P <0. 05 ). Conclusions Emodin induces autophagy in the nasopharyngeal carcinoma CME-1 cells after radiation combined with emodin. Autophagic cell death ( ACD ) may be one of the paths of the radiosensitiza-tion sensitization effect.%目的 研究大黄素在放射增敏过程中对鼻咽癌CNE-1细胞是否产生自噬现象,并探讨其放射增敏作用与自噬相关基因mRNA表达的关系.方法 鼻咽癌细胞分为空白组、大黄素组、照射组和大黄素联合射线增敏组,采用透射电镜观察各组细胞内自噬小体的形成及超微结构的改变,并运用荧光定量PCR方法检测基因ULK1、GABARAPL1、Beclin1、Bcl-2的mRNA表达水平.结果 除空白组外其余各实验组均可观察到自噬小体的形成.与空白组比较,各组ULK1mRNA表达均升高(P<0.05),其中大黄素增敏组表达最高(P<0.01);而Beclin1和Bcl-2 mRNA表达下调;GABARAPL1除大黄素增敏组升高外(P<0.05),其它各组变化不明显.结论 大黄素在鼻咽癌细胞放射增敏过程伴随着自噬产生,自噬性死亡可能是大黄素放射增敏的途径之一.
展开▼
