丙戊酸对人脑胶质母细胞瘤细胞株抑制增殖及诱导凋亡作用研究

摘要

Aim To study the effects of 2-propylpen-tanoic acid ( VPA ) on proliferation and apoptosis in human glioblastoma cell lines in vitro in order to provide a theoretical basis for clinical treatment. Methods 3-(4, 5-Dimethylthiazol-2-yl )-2, 5-diphenyltetrazo-lium bromide ( MTT ) assay was used to determine the cell proliferation. The effecLs on cell cycle arrest and cell apoptosis were observed by flow cylometry , and the expression of acetyl-histone 113 and 114 were detec-ted by Western blot. Results VPA inhibited cell pro-liferation in the glioblastoma cell lines SF295 and U87; G2/M phase arrest was induced by VPA. The expression of aceyl-hisLone 113 and 114 was significantly increased in a time-dependence manner. Apoptosis e-merged after a high concentration treatment with VPA. Conclusion VPA may inhihit tho proliferation of glio-blastoma cell lines, induce cell cycle arrest, and pro-mote apoptosis, which could resuk from ptomoting his-tone acetylation.%目的 研究丙戊酸(2-propylpentanoic acid,VPA)体外对人脑胶质母细胞瘤细胞株增殖抑制、诱导细胞周期阻滞及促进凋亡的作用,为临床治疗提供理论依据.方法 MTT比色法检测VPA对胶质母细胞瘤细胞株的杀伤作用;流式细胞术检测其对细胞周期及凋亡的影响作用;Western blot法检测乙酰化组蛋白H3(acetyl-Histone H3)、乙酰化组蛋白H4(acetyl-histone H4)表达量变化情况.结果 VPA对胶质瘤母细胞瘤细胞株SF295、U87具有抑制增殖作用,诱导细胞周期阻滞于G2 / M 期,乙酰化组蛋白H3、H4表达量呈时间依赖性增加.大剂量可以诱导出现凋亡.结论 VPA能够在体外抑制胶质瘤细胞生长,诱导细胞周期阻滞及促进细胞凋亡,其机制可能与促进组蛋白乙酰化有关.