抗蛇毒IgY对尖吻蝮（Deinagkistrodon acutus）蛇毒主要活性的中和作用研究

摘要

Aim To evaluate the potency of anti-D. acutus venom IgY neutralizing the main activities of D. acutus venom.Methods After mixing the different a-mounts of IgY with snake venom and incubating togeth-er,the main activities of snake venom were assayed by biochemical methods.Results The in vitro assays in-dicated that anti-D.acutus venom IgY obviously neu-tralized the activities of PLA2 ,5′-nucleotidase,hyalu-ronidase,metalloprotease and serine proteinase (fi-brinogenase)in D.acutus venom.Mouse experiments showed that the ED50 value of IgY for mouse was 1 131.09 μg.Conclusion Anti-D.acutus venom IgY antibodies have good effects in neutralizing D.acutus venom without the toxicities themselves.%目的：评价抗尖吻蝮蛇（D．acutus）毒IgY对该蛇毒主要活性的中和能力。方法通过不同浓度IgY与该蛇毒混合孵育后，再用生化方法检测该蛇毒的多种活性。结果体外实验表明，IgY能明显地中和该蛇毒的PLA2、5′-核苷酸酶、透明质酸酶、金属蛋白酶和丝氨酸蛋白酶（纤维蛋白酶）等酶活性；小鼠实验表明，IgY 对小鼠的半有效保护剂量（ED50）为1131.09μg。结论该抗尖吻蝮蛇毒IgY 具有很好的中和活性，且IgY无明显的毒性。