液相色谱/质谱联用技术的新进展和中药研究的相关热点问题

摘要

中药的副作用较少,疗效确切且价格低廉.在中国,中药广泛应用于各种疾病的预防和治疗已经有上千年的历史.然而,中药成分的复杂性却成为其现代化和全球化进程中的瓶颈.本文综述了液相色谱/质谱联用(包括四级质谱、离子阱质谱、飞行时间质谱和杂交质谱)及数据处理技术应用于中药定性、定量研究的新进展.与此同时,本文还论述了与中药研究相关的一些热点问题,如质量控制,指纹图谱,多组分定性、定量,药物代谢动力学及代谢组学.此外,对于液相色谱质谱联用技术应用于复杂组分研究的未来发展方向,如多重离子化质谱策略,多维液相色谱及纳升液相色谱也进行了简述.%Traditional Chinese medicines(TCM)have been widely used for the prevention and treatment of various diseases for rnthousands of years in China,due to their less side-effect,proven efficacy and low cost.But the complexity of the components is still a bottleneck for its modernization and globalization.This paper reviews recent developments in liquid chromatography/mass spectrometry(including quadruple,ion traps,time-of-flight mass analyzers and hybrid instruments)and data processing techniques in the qualitative and quantitative analyses of TCMs.In addition,several hot topics in TCM research such as quality control,fingerprint,multi-component qualification and quantification,pharmacokinetics,and metabolomics are also discussed.Finally,future directions of LC/MS for research on complex systems,such as multiple ionization mass spectrometry strategy,multiple dimensions LC and nanospray LC are also discussed concisely.