中国汉赛巴尔通体分离株的多位点序列分型分析

摘要

To understand the genetic background and epidemiological subtype of Bartonella henselae isolates in China,multilocus sequence typing method was used to analyzed the molecular epidemiological characteristic and phylogenetic relationship for B. henselae. Results showed that 80 B. henselae isolates were belonged to three sequence types (STs). 90％ isolates was ST1 (72/80) and 8. 75％ was ST9 (7/80), while only one strain was belonged to a new type ST-ST30. In addition, all the three STs were belonged to clonal complex 1. Compared with the other reports on B. henselae MLST analysis, the STs were centralized in China, suggesting low diversity and evolution speed among B. henselae isolates in China.%目的 了解中国汉赛巴尔通体的遗传背景和流行的常见亚型.方法 对分离到的汉赛巴尔通体应用多位点序列分型方法(multilocus sequence typing,MLST),分析它们的分子流行病学特征和系统发育情况.结果 80株汉赛巴尔通体一共分为3个序列型(sequence type,ST),其中ST1占90% (72/80),ST9占8.75% (7/80),另一个序列型为仅包含一株细菌的ST30;所有3个序列型均属于克隆群1 (clonal complex 1).结论 与国外汉赛巴尔通体多位点序列分型结果相比,中国的序列型相对集中,说明中国汉赛巴尔通体的变异度和多样性较低,提示其进化相对缓慢.