In order to investigate the value of amplifying Sj26, Sj32 and Sj14-3-3 encoding gene by RT-PCR for diagnosis patients with acute schistosomiasis japonica, the total RNA was extracted from the sera of patients with acute schistosomiasis japonica. The antigen encoding gene of Sj26, Sj32 and Sj14-3-3 were amplified by RT-PCR from the total RNA and identified by 1.2％ Agarose Ⅱ. At the same time, the total RNA were obtained from the sera of patients with paragonimiasis westermani, clonorchiasis sinensis and the normal subjects as the control groups. A 400bp Sj14-3-3 encoding gene was obtained by RT-PCR from the 50 schistosomiasis japonica patients, but the encoding gene of Sj26 and Sj32 weren't obtained, and the control groups were negative. The sensitivity and specialty were both 100％ by RT-PCR with Sj14-3-3. There was no cross reaction between clonorchiasis sinensis and paragonimiasis westermani. It's indicated that the amplification of Sj14-3-3 encoding gene might be used for the gene diagnosis of patients with acute Schistosomiasis japonica.%目的 探讨RT-PCR扩增Sj26、Sj32和Sj14-3-3抗原编码基因用于急性日本血吸虫病患者的诊断价值.方法 从急性日本血吸虫病患者血清中提取总RNA,利用RT-PCR方法扩增Sj26、Sj32和Sj14-3-3抗原编码基因,用1.2%琼脂糖凝胶电泳进行鉴定,同时以华支睾吸虫病患者血清、卫氏并殖吸虫病患者血清和正常人血清总RNA作为对照.结果 50份急性日本血吸虫病血清均通过RT-PCR扩增出了400bp的Sj14-3-3抗原编码基因片段,但未扩增出676bp的Sj26和1 270bp的Sj32抗原编码基因片段;对照组呈阴性反应.RT-PCR扩增Sj14-3-3抗原编码基因片段敏感性和特异性均为100%,与华支睾吸虫病和卫氏并殖吸虫病患者血清未见交叉反应.结论 RT-PCR扩增Sj14-3-3抗原编码基因可用于急性日本血吸虫病的基因诊断.
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