2. Infectious Pancreatic Necrosis Virus Detection Program (IPNV), including RT-PCR and restriction enzyme digestion to amplify VP2 coding fragments to detect attenuated and toxic IPNV strains.

摘要

Procedure for the detection of Infectious pancreatic necrosis virus (IPNV) comprising Infect host cells with IPNV, extract DNA, Amplifying a fragment of the complementary DNA.Purify the DNA, Cloning and Transformation of competent cells, the DNA Extraction and PCR Detection plasmidial variant of IPNV.