Skp2和PTEN在上皮性卵巢肿瘤组织中的表达及临床意义

摘要

目的 研究S期激酶相关蛋白2(Skp2)和PTEN蛋白在上皮性卵巢肿瘤组织中表达,探讨其临床病理学意义及在肿瘤发生发展中作用.方法 收集129例上皮性卵巢肿瘤石蜡标本(良性35例、交界性32例、恶性62例),免疫组化法检测Skp2及PTEN蛋白的表达水平,分析两者表达、临床病理参数关系及其相互关系.结果 ①Skp2在良性及交界性肿瘤中表达阴性,分别为(0/35,0/32),在卵巢癌中阳性率40.32%(25/62),其在卵巢癌表达水平明显高于良性和交界性肿瘤(χ2值分别为8.25、6.72,均P<0.05);②PTEN蛋白在良性及交界性肿瘤阳性率分别为82.86%(29/35)、59.38%(19/32),在卵巢癌阳性率40.32%(25/62),明显低于良性和交界性肿瘤(χ2值分别为11.15、9.23,均P<0.05);③Skp2、PTEN蛋白在卵巢癌的表达在不同年龄、是否绝经及不同组织学类型中无显著性差异(χ2值分别为1.01、0.04、0.21,P>0.05).Skp2在卵巢癌Ⅲ~Ⅳ期、中低分化组及有淋巴结转移组表达明显高于Ⅰ~Ⅱ期、高分化组及无淋巴结转移组(χ2值分别为10.05、11.21、8.54,均P<0.05),PTEN在卵巢癌Ⅲ~Ⅳ期、中低分化组及有淋巴结转移组表达低于Ⅰ~Ⅱ期、高分化组及无淋巴结转移组(χ2值分别为8.23、12.15、7.65,均P<0.05);④Skp2和PTEN蛋白在卵巢癌中的表达呈负相关(r=-0.321,P<0.05).结论 Skp2表达上调与PTEN下调可能在上皮性卵巢肿瘤的发生、发展中起重要作用.检测Skp2和PTEN表达水平可能对卵巢癌预后分析有一定指导意义.%Objective To investigate the expressions of Skp2 and PTEN in epithelial ovarian tumors and explore their clinicopathologic significance as well as the role in genesis of tumor.Methods Skp2 and PTEN protein expressions were examined by immunohistochemistry in 129 epithelial ovarian tumors (35 cases of adenomas, 32 cases of tumors of borderline, and 62 cases of denocarcinomas.The expressions of two, clinicopathologic parameters and their relationship were analyzed.Results Positive rate of Skp2 both in ovarian adenoma and borderline adenoma was 0 (0/35, 0/32), and the positive rate in ovarian adenocarcinoma was 40.32%(25/62), which was significantly higher than in ovarian adenoma and borderline adenoma (χ2 value was 8.25 and 6.72, respectively, both P<0.05).Positive rate of PTEN in ovarian adenoma and borderline adenoma was 82.86% (29/35) and 59.38% (19/32), respectively, and that in ovarian adenocarcinoma was 40.32% (25/62), which was significantly lower than in ovarian adenoma and borderline adenoma (χ2 value was 11.15 and 9.23, respectively, both P<0.05).The expression levels of Skp2 and PTEN were independent of age, menopause and histological type (χ2 value was 1.01, 0.04 and 0.21, respectively, all P>0.05).The expression of Skp2 was remarkably higher in ovarian cancer atⅢ-Ⅳstage, moderately and poorly differentiated group and lymph node metastasis group than in ovarian cancer atⅠ-Ⅱstage, higher differentiated group and no lymph node metastasis group (χ2 value was 10.05, 11.21 and 8.54, respectively, all P<0.05), but PTEN was lower (χ2 value was 8.23, 12.15 and 7.65, respectively, all P<0.05).The expression of Skp2 was negatively correlated with that of PTEN (r=-0.321, P<0.05).Conclusion Up-regulation of Skp2 and down-regulation of PTEN may play an important role in the genesis and development of epithelial ovarian tumors.Detecting the expression levels of Skp2 and PTEN might have value on analyzing the prognosis of ovarian cancer.