不同动物源生产用多杀性巴氏杆菌外膜蛋白H基因序列分析

摘要

为了解国内不同动物源生产用多杀性巴氏杆菌外膜蛋白H基因的变异情况,采用PCR方法对16株不同动物源生产用多杀性巴氏杆菌的ompH基因进行扩增测序和分析.结果显示:16株菌株的ompH基因开放阅读框在1002～1056 bp之间;信号肽为N端20个氨基酸残基,成熟蛋白氨基酸残基数量在313～331 aa之间,氨基酸同源性为82.1％ ～ 100％;基于OmpH氨基酸序列的系统发育树中鸡源菌株(荚膜A型)、猪源和牛源菌株(荚膜B型)分别在不同的分支.序列分析结果表明,ompH基因序列差异与荚膜型之间存在相关性,而与毒力大小无相关性.%In order to understand the variation of outer membrane protein H gene,the ompH gene of 16 P.multocida strains were examined and analysed by PCR method.The results showed that the open reading frames of ompH gene were from 1002 to 1056 bp.The predicted mature proteins were composed of 313 to 331 amino acids except the signal peptide including 20 amino acid residues in N terminal.The alignment data indicated that the homology of amino acid were from 82.1％ to 100％.The phylogenetic tree constructed by the OmpH protein showed that the strains isolated from avian (capsular type A),swine and cattle (capsular type B) were in different branches.The relationship were found between the sequence divergence of ompH gene and the capsular type,but not with the virulence.