Mallotus furetianus Tea was extracted using petroleum ether and 95% ethanol respectively to have fat soluble components, monosaccharide, oligosaccharides, and glycosides removed. And then the tea polysaccharides were extracted from Mallotm furetianus by water extraction and ethanol deposition method. The Sevag reagent and protein enzyme were used to get rid of the protein in the tea polysaccharides. Glucose was used as the standard and the amount of tea polysaccharides was determined by anthrone -H2SO4 colorimetric method. The effect of ethanol concentration on tea polysaccharides deposition was also examined. The results showed that the belter method on dispositing protein was combining Sevag reagent with protein enzyme. 80% ethanol concentration presented the best effect on polysaccharides deposition. The content of tea polysacchrides in Mallotus furetianus Tea was 1.012% when the determined wavelength was 604 nm, and the recovery rate was 96.7%, and the relative standard deviation RSD 2.45%, the liquid was steady in 4 hours. This tea polysaccharides determination method is rapid, accurate and reproducible.%用石油醚和95％乙醇分别浸提山苦茶叶,以去除其中脂溶性成分和单糖、低聚糖、苷类等杂质,再用水提醇沉法提取分离山苦茶多糖,最后采用Sevag试剂法和蛋白酶酶解法去除茶多糖中的蛋白质.以葡萄糖为标准,蒽酮-硫酸比色法测定山苦茶多糖含量.考察了乙醇浓度对山苦茶多糖沉淀量的影响.结果表明,采用Sevag试剂法和蛋白酶酶解法相结合去除蛋白质效果较好.采用80％乙醇浓度沉淀山苦茶多糖效果最佳,测定波长为604 nm,山苦茶中多糖含量为1.012％,方法回收率96.7％,RSD为2.45％,多糖提取液4h内显色稳定.该试验方法测定茶多糖准确快速,显色稳定且精密度好.
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