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Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea

机译:鹰山云雾茶多糖的化学组成及抗氧化活性

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摘要

The study was designed to investigate the chemical composition and antioxidant activities of polysaccharides from Yingshan Cloud Mist Tea. The chemical composition of green tea polysaccharides (GTPS) was analyzed by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), thermogravimetric (TGA), gas chromatograph (GC), and high-performance gel-permeation chromatography (HPGPC). Then, the antioxidant activities in vitro of GTPS, effects of GTPS on body weight, and the antioxidant activities in chickens were studied. The results showed that GTPS were composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) in a molar ratio of 11.4 : 26.1 : 1.9 : 3.0 : 30.7 : 26.8 and the average molecular weight was 9.69 × 104 Da. Furthermore, GTPS exhibited obvious capacity of scavenging DPPH radical, hydroxyl radical, and superoxide radical and enhanced the ferric-reducing power in vitro. Last, GTPS significantly increased the body weight of chickens, enhanced the T-AOC, SOD, and GSH-Px level, and decreased the content of MDA in chickens. The results indicated that GTPS might be a kind of natural antioxidant, which had the potential application in feed industry.
机译:本研究旨在调查鹰山云雾茶多糖的化学组成和抗氧化活性。通过傅立叶变换红外光谱(FT-IR),扫描电子显微镜(SEM),热重分析(TGA),气相色谱仪(GC)和高效凝胶渗透色谱法分析了绿茶多糖(GTPS)的化学成分(HPGPC)。然后,研究了GTPS的体外抗氧化活性,GTPS对体重的影响以及鸡的抗氧化活性。结果表明,GTPS由鼠李糖(Rha),阿拉伯糖(Ara),木糖(Xyl),甘露糖(Man),葡萄糖(Glu)和半乳糖(Gal)组成,摩尔比为11.4:26.1:1.9:3.0 :30.7:26.8,平均分子量为9.69×10 4 Da。此外,GTPS表现出明显的清除DPPH自由基,羟基自由基和超氧化物自由基的能力,并增强了体外的铁还原能力。最后,GTPS显着增加了鸡的体重,提高了T-AOC,SOD和GSH-Px水平,并降低了鸡的MDA含量。结果表明,GTPS可能是一种天然抗氧化剂,在饲料工业中具有潜在的应用前景。

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