一种适合油棕不同组织 RNA 提取的方法

摘要

High quality of RNA is the important prerequisite and guarantee to basic research related to oil palm fruit development in molecular biology. RNA extracting effects of both Trizol reagents and MRIP (Methods for RNA Isolation from Palms) method developed by our laboratory previously were compared among four oil palm tissues, i.e. mature mesocarp, endosperm, embryo and germinated plumule. MRIP can get good quality RNA.The RNA content of plumule is as high as 2 139.0 ng/μL, while that of endosperm is only 1 083.6 ng/μL. MRIP is generally better than Trizol method in respect of the integrality, content and purity of extracted RNA, which Suitable for oil palm and palm plants RNA extraction.%获得高质量的 RNA 是开展油棕果实发育相关分子生物学基础研究的重要前提和保障。以油棕成熟果肉、胚乳、胚和胚芽4种组织为材料，分别采用 Invitrogen 公司的 Trizol 试剂和 MRIP 法提取总 RNA，并比较其提取效果。结果表明： MRIP 法提取的 RNA 质量较好，其中胚芽中的总 RNA 提取量高达2139.0 ng/μL，而胚乳中的含量相比较低，仅为1083.6 ng/μL； MRIP 法的总体提取效果优于 Trizol 试剂，包括 RNA 完整性、含量、纯度等，适用于油棕及棕榈科植物总 RNA 提取。