可注射藻酸盐支架与人骨髓基质干细胞的体外复合培养

摘要

背景:目前可注射组织工程骨的研究主要限于动物实验,若人骨髓基质干细胞与藻酸盐生物相容性良好,可注射组织工程骨将是极具前途的临床治疗手段.目的:体外观察人骨髓基质干细胞与可注射支架藻酸钙凝胶的生物相容性.方法:实验组将第2代人骨髓基质干细胞与藻酸钙凝胶复合培养,对照组单纯接种骨髓基质干细胞.倒置相差显微镜、扫描电镜观察各组细胞形态及增殖情况,MTT法半定量检测细胞增殖情况.结果与结论:倒置显微镜下见实验组细胞生长良好,与对照组无明显差异.扫描电镜见骨髓基质干细胞在藻酸钙表面贴附、增殖良好,第6天时细胞已跨越微孔表面或向孔内生长.MTT法显示与对照组相比,实验组细胞增殖能力不受影响.结果初步表明藻酸钙与人骨髓基质干细胞体外生物相容性较好.%BACKGROUND: Injectable tissue-engineered bone is limited to animal experiments. If the human bone marrow stromal stem cells have a good biocompatibility with alginate, the injectable tissue engineered bone will be a very promising clinical treatment. OBJECTIVE: To study the biocompatibility of human bone marrow stromal stem cells and injectable scaffold of calcium alginate gels in vitro.METHODS: The second passage human bone marrow stromal stem cells were co-cultured with calcium alginate gels. Bone marrow stromal stem cells cultured with no material served as controls. The morphology and proliferation of the cells were observed by inverted phase contrast microscope and scanning electron microscope. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to semi-quantitatively evaluate the cell proliferation. RESULTS AND CONCLUSION: The human bone marrow stromal stem cells co-cultured with calcium alginate gels grew well under inverted phase contrast microscope, and no significant difference was found between calcium alginate gels group and control group. Under scanning electron microscope, human bone marrow stromal stem cells adhered well to the calcium alginate gels and proliferated obviously. MTT results showed that the cell number had no significant difference between the two groups. It is suggested that calcium alginate gels have good biocompatibility with human bone marrow stromal stem cells.