骨组织块法和酶消化法联合培养人成骨细胞

摘要

BACKGROUND: It is difficult to culture osteoblasts, and different in cell number, purity, proliferation and differentiation activity obtained by different culture methods.OBJECTIVE: To investigate the ideal culturing method of the human osteoblasts in vitro.METHODS: Human osteoblasts were isolated from trabecular bone of ilium by bone tissue and enzymatic digestion methods.RESULTS AND CONCLUSION: The isolated and cultured human osteoblasts were in good condition and had a high purity. The cells were adherent growth, diversification in morphology, and mostly showed polygonal, spindle-shaped, triangular, and the cytoplasm was abundant and the spurt was stretched out. It has the typical characteristics of osteoblasts. The cells proliferation was well, alkaline phosphatase staining and mineralized nodules alizarin red staining was positive. We successfully cultured osteoblasts in vitro from trabecular bone of ilium by bone tissue and enzymatic digestion methods; it indicated that was the ideal method to culture osteoblasts.%背景:成骨细胞培养难度大,不同培养方法得到的成骨细胞数量、纯度、增殖及分化活性各有区别.目的:探讨理想的人成骨细胞体外培养的方法.方法:取人髂骨松质骨,同时结合骨组织块法和酶消化法分离培养人成骨细胞.结果与结论:分离培养的人成骨细胞生长状态良好,纯度较高.细胞贴壁生长,形态多样化,多呈多边形、纺锤型、梭形、三角形,胞浆丰富向外伸展出生长突,具有典型的成骨细胞形态特征;细胞增殖良好,碱性磷酸酶染色及矿化结节茜素红染色阳性.说明联合骨组织块法和酶消化法可成功分离培养人成骨细胞,是进行人成骨细胞体外培养较为理想的方法.