利用微阵列芯片技术探究脊髓损伤的分子机制★

摘要

背景：脊髓损伤后一系列生物过程变化的分子机制尚不明确。目的：分析利用微阵列芯片技术观察脊髓损伤后的基因表达变化的国际研究进展。方法：应用计算机检索Elsevier数据库和Web of Knowledge数据库中1972年1月至2012年11月关于微阵列芯片技术和脊髓损伤方面的文章，在标题和摘要中以“microarray, spinal cord injury, gene expression”为检索词进行检索。选择文章内容与利用微阵列技术探究脊髓损伤分子机制相关，同一领域文献则选择近期发表在较高水平杂志中的文章。根据纳入标准选择56篇文献进行综述，同时参考了两部中文著作。结果与结论：利用在分子研究基础上建立起来的微阵列芯片技术能够很好的检测从急性损伤期到后期胶质瘢痕形成过程中的基因表达变化，进而能够寻找相关的信号通路及转录因子。该技术不仅对今后的分子研究起到指导作用，而且可从基因层面为脊髓损伤的治疗寻找合适的靶点。文章分析了脊髓损伤的病理生理学过程，提出了实验设计及微阵列芯片检测技术上的革新、数据分析方法上的改进，并评价了微阵列芯片帮助寻找有效治疗靶点的能力。%　　BACKGROUND: The molecular mechanisms of different biological processes after spinal cord injury are not clear. OBJECTIVE: To analyze the research advance of the gene expression change after spinal cord injury by using microarray technology. METHODS: Elsevier and Web of Knowledge databases were retrieved by computer with key words of “microarray, spinal cord injury, gene expression” to search papers about microarray and spinal cord injury published between January 1972 and November 2012. Related papers addressing the molecular mechanism of spinal cord injury by using microarray technology were selected. According to inclusion criteria, 56 literatures were selected in this study. Two Chinese writings were also consulted. RESULTS AND CONCLUSION: The change of gene expression from acute phase to chronic phase fol owing spinal cord injury could be detected by using microarray technology which is established on molecular research, and then, the relevant signaling pathway and transcription factors could be found. This technology not only plays a guiding role in further molecular research, but also can be used to find the suitable therapeutic targets of spinal cord injury on genetic level. The research advance of pathophysiology and the gene expression after spinal cord injury by using microarray technology was reviewed in this paper. The technological innovation of experimental design and microarray detection, the improvement of data analysis methods were put forward. The power of microarrays in finding potential therapeutic targets was also evaluated.