骨髓间充质干细胞干预再生障碍性贫血患者树突状细胞的成熟分化★

摘要

背景：骨髓间充质干细胞对再生障碍性贫血患者T细胞增殖的影响国内仅见少量报道，而骨髓间充质干细胞是否通过调节树突状细胞来影响再生障碍性贫血患者T细胞的增殖，国内未见报道，其机制值得深入研究。目的：观察骨髓间充质干细胞对再生障碍性贫血患者树突状细胞的免疫调节作用。方法：将培养第5天的再生障碍性贫血患者外周血单个核细胞来源的树突状细胞与第3代健康人骨髓间充质干细胞混合培养，加入脂多糖、肿瘤坏死因子促树突状细胞成熟，应用流式细胞仪检测骨髓间充质干细胞与未成熟、成熟树突状细胞共培养前后树突状细胞表面标志表达。结果与结论：未成熟的树突状细胞在脂多糖的刺激诱导下与骨髓间充质干细胞共培养前后，树突状细胞表面标志CD14，CD1a，CD83，CD80表达无变化(P>0.05)；成熟树突状细胞与骨髓间充质干细胞共培养前后，树突状细胞表面标志CD14，CD1a，CD83，CD80表达降低(P<0.05)。结果说明骨髓间充质干细胞可抑制再生障碍性贫血患者单核细胞来源的树突状细胞的发育和成熟，进而发挥调节再生障碍性贫血患者的免疫作用。%BACKGROUND:There are few reports about the influence of bone marrow mesenchymal stem cel s on T cel proliferation in aplastic anemia patients in China. Besides, no study is reported in China on the topic that whether bone marrow mesenchymal stem cel s affect the T cel proliferation in aplastic anemia patients by dendritic cel regulation. Therefore, its mechanism deserves further investigations. OBJECTIVE:To explore effects of human bone marrow mesenchymal stem cel s on dendritic cel immune regulation in patients with aplastic anemia. METHODS:Human peripheral blood mononuclear cel s-derived dendritic cel s cultured for 5 days were co-cultured with human bone marrow mesenchymal stem cel s at passage 3. Lipopolysaccharide and tumor necrosis factor were added to promote the maturation of dendritic cel s. Flow cytometry was used to detect the expression of dendritic cel surface markers before and after coculture of bone marrow mesenchymal stem cel s and immature and mature dendritic cel s. RESULTS AND CONCLUSION:Under the induction of lipopolysaccharide, prior to and fol owing the coculture of immature dendritic cel s and bone marrow mesenchymal stem cel s, no changes of the expression of CD14, CD1a, CD83 and CD80 were observed (P>0.05). Prior to and fol owing the coculture of mature dendritic cel s and bone marrow mesenchymal stem cel s, the expression of CD14, CD1a, CD83 and CD80 was decreased (P<0.05). Results suggested that bone marrow mesenchymal stem cel s can inhibit the development and maturation of dendritic cel s derived from monocytes of aplastic anemia patients, and exhibit immunoregulatory effects in patients with aplastic anemia.