背景:利用人牙龈成纤维细胞和细胞因子相结合是修复牙周组织缺损的一个新的研究热点。 目的:观察骨形态发生蛋白2联合地塞米松对体外培养的人牙龈成纤维细胞的细胞增殖及成骨分化的影响。方法:培养至第3代的人牙龈成纤维细胞分5组培养:DMEM组、基础骨诱导组、基础骨诱导+地塞米松组、骨诱导+骨形态发生蛋白2组,骨诱导+地塞米松+骨形态发生蛋白2组。MTT法检测各组细胞增殖情况,碱性磷酸酶染色、茜素红染色及反转录多聚酶链反应检测各组细胞的成骨分化情况。 结果和结论:人牙龈成纤维细胞具有成骨分化的潜能,骨形态发生蛋白2、地塞米松对人牙龈成纤维细胞的细胞增殖无明显影响,单独及联合应用地塞米松、骨形态发生蛋白2能够促进人牙龈成纤维细胞的碱性磷酸酶活性和钙结节的形成,其中两者联合的作用更强,而且后者能明显促进成骨相关基因的表达,提示两者联合更有效地促进人牙龈成纤维细胞成骨分化。%BACKGROUND:It is novel research field in repairing periodontal tissue defects by human gingival fibroblasts combined with cytokines. OBJECTIVE:To investigate the effect of bone morphogenetic protein-2 combined with dexamethasone on the proliferation and osteogenic differentiation of human gingival fibroblasts. METHODS:Human gingival fibroblasts at passage 3 were divided into five groups: DMEM, basal osteogenic medium, basal osteogenic medium and dexamethasone, basal osteogenic medium and bone morphogenetic protein-2, basal osteogenic medium, dexamethasone and bone morphogenetic protein-2 groups. Cel proliferation was detected by MTT. Osteogenic differentiation was examined by alkaline phosphatase activity, alizarin red staining and reverse transcription PCR. RESULTS AND CONCLUSION:Human gingival fibroblasts had the potency of osteogenic differentiation. Dexamethasone and bone morphogenetic protein-2 had no significant influence on the proliferation of human gingival fibroblasts. Alkaline phosphatase activity and calcium deposition were promoted by dexamethasone or bone morphogenetic protein-2, especialy by both together. Combination of dexamethasone and bone morphogenetic protein-2 can promote the expression of osteogenic related-genes, which enhances the osteogenic differentiation of human gingival fibroblasts effectively.
展开▼