BACKGROUND:The first step of tissue engineering research is to produce a large number of seed cels with good biological activity, fast proliferation and high purity. OBJECTIVE:To explore the biological characteristics of rat bone marrow mesenchymal stem cels isolated and cultured by the whole bone marrow adherence method. METHODS: Rat bone marrow mesenchymal stem cels were isolated and cultured, and then passage 3 cels were enroled to draw a cel growth curve. Morphological changes of cels at different passages were observed. Cels in passage 2 and passage 3 were characterized by their surface marker expressions of CD29, CD90, CD34 and CD45. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cels at passages 1-3 exhibit classic morphology of fibroblast-like cels and presented plastic-adherent property. Phenotypes of P2 generation were detailed as: expressions of CD29, CD90, CD34, CD45 were 91.5%, 96.2%, 1.3%, and 8.0%, respectively. Phenotypes of passage 3 cels were detailed as: expressions of CD29, CD90, CD34, CD45 were 98.2%, 98.1%, 0.6%, 2.0%, respectively. The cel growth curve indicated the cel proliferation reached the peak at an inoculation concentration of 8×107/L. Overal, bone marrow mesenchymal stem cels at passage 3 with good viability, rapid proliferation and high purity can be used as seed cels for tissue engineering research.%背景：进行组织工程研究的首要环节是培养出大量生物活性好、增殖速度快、细胞纯度高的种子细胞。目的：分析全骨髓贴壁法培养大鼠骨髓间充质干细胞的生物学特性。 方法：采用全骨髓贴壁法分离、培养大鼠骨髓间充质干细胞，绘制第3代细胞生长曲线，观察各代细胞形态学变化，流式细胞分析法测定第2代、第3代细胞表面标志物(CD29、CD90、CD34、CD45)的表达。 结果与结论：经全骨髓贴壁法培养3代以内骨髓间充质干细胞均具有典型成纤维样细胞形态而且贴壁生长；第2代骨髓间充质干细胞表型CD29、 CD90、CD34、CD45表达分别为91.5%，96.2%，1.3%，8.0%，第3代骨髓间充质干细胞分别为98.2%，98.1%，0.6%，2.0%；细胞生长曲线结果表明，以细胞浓度8×107 L-1接种，细胞增殖速度快，获得的细胞总数多。综合形态学及细胞鉴定结果，第3代大鼠骨髓间充质干细胞活性好、增殖速度快、细胞纯度高，适合作为组织工程研究的种子细胞。
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