BACKGROUND:As the main component of articular cartilage, type II col agen can induce bone marrow mesenchymal stem cel s to differentiate into the cartilage. However, there is no uniform standard for the preparation of type II col agen hydrogel and its usage in the repair of sports-induced cartilage injury. OBJECTIVE:To investigate the effect of type II col agen hydrogel-cel complexes in the repair of cartilage injury. METHODS:After modeling, 30 New Zealand rabbits with cartilage injury were randomized into two groups (n=15 per group):type II col agen hydrogel-bone marrow mesenchymal stem cel complexes were implanted into the injured site of rabbits in experimental group, while only type II col agen hydrogel implanted in control group. Histomorphology observation was performed by hematoxylin-eosin and toluidine blue staining after 4 and 8 weeks after implantation. RESULTS AND CONCLUSION:In the experimental group, there were inflammatory cel s infiltrated at the injured site, most of which were macrophages and only a smal amount of which were neutrophils under hematoxylin-eosin staining, at 4 weeks after implantation, while toluidine blue staining showed no positive. At 8 weeks after implantation, a large amount of chondrocytes proliferated at the injured site that was repaired by chondroblasts and myotubes as wel as new vessels under hematoxylin-eosin staining, and toluidine blue staining showed the injured tissues were similar to normal tissues. In the control group, at 4 weeks after implantation, obvious interstitial edema existed, whereas skeletal muscle cel s disappeared around the injured site, and a lot of inflammatory cel s infiltrated. Several chondroblasts formed at 8 weeks, accompanied by increased fibrous tissues. Moreover, toluidine blue staining always showed no positive in the control group. To conclude, the type II col agen hydrogel-cel complex has better chondrogenic ability that can be used for cartilage repair.%背景：体外实验显示，Ⅱ型胶原能诱导骨髓间充质干细胞成软骨分化，但有关Ⅱ型胶原水凝胶制备及其在软骨运动损伤中的修复尚缺乏报道。 目的：观察Ⅱ型胶原水凝胶-细胞复合物在软骨损伤修复中的作用。 方法：取30只新西兰大白兔，建立软骨损伤模型，造模后随机分为2组，每组15只，实验组于软骨损伤处植入Ⅱ型胶原水凝胶-骨髓间充质干细胞复合物，对照组植入Ⅱ型胶原水凝胶。植入后4，8周，取材软骨损伤部位，进行苏木精-伊红染色及甲苯胺蓝染色。 结果与结论：①实验组：植入后4周，苏木精-伊红染色结果可见炎性细胞浸润，以巨噬细胞为主，存在少量中性粒细胞；甲苯胺蓝染色未见阳性。植入后8周，苏木精-伊红染色下可见大量软骨细胞增殖，有成软骨和肌管形成，并且可见血管再生；甲苯胺蓝染色接近正常组织；②对照组：植入后4周，苏木精-伊红染色可见损伤周围间质水肿明显，损伤骨骼肌细胞核消失，存在大量炎性细胞浸润；甲苯胺蓝染色未见阳性。植入后8周，苏木精-伊红染色下可见少许成软骨细胞形成，纤维组织增多；甲苯胺蓝染色未见阳性；③结果表明：Ⅱ型胶原水凝胶-细胞复合物修复软骨损伤具有良好的成软骨能力。
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