血管内皮祖细胞对共植入缺血再灌注模型大鼠神经干细胞增殖、凋亡和血管重建的影响

摘要

BACKGROUND:Neural stem cel (NSC) transplantation is a common method for various ischemicencephalopathies, but inability to survive in the transplantation region limits its further use in clinical practice. OBJECTIVE:To explore the effect of vascular endothelial progenitor cel s (VEPCs) on the proliferation and apoptosis of co-cultured NSCs as wel as vascular remodeling in rats with ischemia reperfusion injury. METHODS:125 Sprague-Dawley rats were randomly divided into five groups, 25 rats in each group, including sham operation, ischemia, NSCs, co-culture, and VEPCs groups. Rat models of ischemia reperfusion injury were made in al groups except for the sham operation group, fol owed by corresponding interventions. The proliferation and apoptosis of neural stem cel s were detected, and vascular remolding in the ischemic region was observed in each group. RESULTS AND CONCLUSION:At different time points after transplantation, BrdU positive cel s were not observed in VEPCs, ischemia and sham operation groups;the number of BrdU positive cel s in the co-culture group was significantly higher than that in the NSCs group (P<0.05);BrdU+/Caspase-3+cel were observed in both co-culture and NSCs groups, and the apoptosis rate of the co-culture group was significantly lower than that in the NSCs group (P<0.05);there were new blood vessels in al the groups except for the sham operation group, and the number of new bone vessels was highest in the co-culture group. To conclude, our experimental results show that VEPCs promotes the proliferation of co-cultured NSCs, inhibits cel apoptosis and and promote angiogenesis in the ischemic penumbra of rats with ischemia reperfusion injury.%背景：神经干细胞移植在缺血性脑血管疾病治疗中的作用已经明确，但细胞不能在移植局部存活等问题限制了进一步的应用。目的：探讨血管内皮祖细胞对共植入缺血再灌注模型大鼠神经干细胞增殖、凋亡和血管重建的影响。方法：125只SD大鼠随机分为5组，假手术组、缺血对照组、神经干细胞组、神经干细胞+血管内皮祖细胞组、血管内皮祖细胞组，每组25只。线栓法制备缺血再灌注大鼠模型，并进行分组干预。移植后1，2，3，4，5周，检测各组神经干细胞增殖、凋亡以及缺血区血管新生情况。结果与结论：①移植后不同时间点，血管内皮祖细胞组、缺血对照组、假手术组均未观察到BrdU阳性细胞。神经干细胞+血管内皮祖细胞组的BrdU阳性细胞数显著高于神经干细胞(P<0.05)；②移植后不同时间点，神经干细胞+血管内皮祖细胞组和神经干细胞组可观察到BrdU+/Caspase-3+双标阳性细胞。神经干细胞+血管内皮祖细胞组的细胞凋亡率显著低于神经干细胞组(P<0.05)；③移植后不同时间点，假手术组均未见新生血管，其他4组均出现新生血管。神经干细胞+血管内皮祖细胞组的血管新生数量显著多于其他各组(P<0.05)；④结果表明，血管内皮祖细胞可以促进共植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖，抑制细胞凋亡，并促进缺血区血管新生。