人颞叶内侧癫痫海马组织星形胶质细胞Syntrophin的表达变化

摘要

目的 观察人颞叶内侧癫痫(MTLE)海马组织星形胶质细胞syntrophin的表达变化.方法 2015年4月至2016年7月,17例癫痫患者手术切除的海马组织,依据苏木素-伊红(HE)染色及胶质原纤维酸性蛋白、神经元核抗原免疫组织化学染色结果,分为MTLE组13例和非MTLE组4例.免疫荧光双重组织化学法及免疫荧光组织化学法观察syntrophin的表达.结果 MTLE组星形胶质细胞大量增生,神经元明显减少.syntrophin在星形胶质细胞膜及足突处表达.与非MTLE组相比,MTLE组syntrophin在血管周围星形胶质细胞足突处分布减少,在其他部位分布增加.图像分析显示,与非MTLE组相比,MTLE组syntrophin蛋白量显著增多(t=5.421,P<0.001).结论 海马组织syntrophin在星形胶质细胞上的分布及其整体表达变化可能与MTLE发生相关.%Objective To investigate the expression changes of astrocytic syntrophin in hippocampus from human mesial temporal lobe epilepsy (MTLE). Methods From April, 2015 to July, 2016, 17 cases of hippocampus, collected from temporal lobectomy, were divided into MTLE group (n=13) and non-MTLE group (n=4) according to hematoxylin and eosin staining, glial fibrillary acidic protein and neuronal nu-clei immunohistochemical staining. Immunofluorescence double labeling and immunofluorescence histochemistry were used to observe the expression of syntrophin. Results The proliferation of astrocytes increased and neurons reduced in the hippocampus of MTLE group. Syntro-phin was found in the membrane and foot processes of astrocyte, that was enriched along perivascular astrocyte end-feet domain in non-MTLE group, but lost in MTLE group. While the whole expression of syntrophin was more in MTLE group than in non-MTLE group (t=5.421, P<0.001). Conclusion The distribution of syntrophin in hippocampus astrocytes may be related to the development of MTLE.