人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4和内向整流性钾离子通道4.1的再分布

摘要

目的 研究人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4(AQP4)和内向整流性钾离子通道4.1(Kir4.1) 的分布.方法 对10 例颞叶内侧癫痫(MTLE)和6 例非颞叶内侧癫痫(non-MTLE)手术切除海马组织,应用光镜及透射电镜观察组织学及超微结构,免疫荧光组织化学法观察星形胶质细胞AQP4 和Kir4.1 的分布.结果 MTLE 海马组织星形胶质细胞大量增生伴明显肿胀,神经元显著固缩.non-MTLE 海马组织中,AQP4 和Kir4.1 在星形胶质细胞血管周围足突(pAST-ef)分布多于其他部位,呈现极性分布特点;而在MTLE 海马组织中,AQP4 和Kir4.1 在pAST-ef 分布减少,其他部位分布增加,极性分布改变.结论 海马组织星形胶质细胞上AQP4 和Kir4.1 极性分布变化可能与颞叶内侧癫痫发生相关.%Objective To investigate the distribution of aquaporin 4 (AQP4) and inwardly rectifying potassium channel 4.1 (Kir4.1) in the astrocytes from human mesial temporal lobe epilepsy (MTLE). Methods Hippocampal specimens, including 10 cases of MTLE and 6 cases of non-MTLE, were observed under optical and transmission electron microscopy. The distribution of AQP4 and Kir4.1 in astrocytes was investigated with immunoflurescence. Results Compared with non-MTLE hippocampus, the main structural changes of MTLE included remarkable hyperplasia astrocytes, serious swelling astrocytes and distinguished astrophy neurons. In non-MTLE hippocampus, immunoflurescence signals of AQP4 and Kir4.1 were enriched along perivascular astrocyte end-feet domain. However, it reveals significant loss of AQP4 and Kir4.1 in perivascular astrocyte end-feet domain in MTLE. Conclusion Loss of perivascular AQP4 and Kir4.1 in the human MTLE may help to understand the roles of astrocyte in MTLE.