量子点免疫荧光技术检测EMMPRIN、MMP-2和P53蛋白在人肺癌组织中的表达意义

摘要

目的:本研究利用新型免疫荧光标记试剂-量子点(QDs)结合组织芯片技术检测细胞外基质金属蛋白酶诱导因子(EMMPRIN/CD147)、基质金属蛋白酶2(MMP-2)和P53 蛋白在人肺癌组织中的表达,并探讨EMMPRIN与肺癌恶性生物学行为的关系.方法:利用QDs免疫荧光组织化学(QDs-IHC)技术分别检测人肺癌组织芯片中EMMPRIN、MMP-2和P53蛋白的表达,并利用QDs免疫荧光双标法同时检测了EMMPRIN与P53蛋白的共表达.结果:EMMPRIN、MMP-2和P53蛋白表达在肺癌组织的阳性率分别为:70.00%、77.14%和72.86%,与非癌变肺组织相比,差异均显著(P＜0.05);而且与肺癌的TNM分期、淋巴结转移均显著相关(P＜0.05),但与其它的临床病理特征均明显无关(P＞0.05).EMMPRIN和MMP-2、P53蛋白表达之间均呈显著正相关(P＜0.01).结论:EMMPRIN、MMP-2和P53蛋白均与肺癌的发生有关,EMMPRIN可能与MMP-2、P53协同促进肺癌的恶性进展.%AIM : To investigate the expression of extracellular matrix metalloproteinase inducer ( EMMPRIN/CD147 ), matrix metalloproteinase2 ( MMP - 2 ) and P53 proteins in human lung cancer tissues and to explore the relationship between EMMPRIN protein and malignant biological behaviour of lung cancer.METHODS : Fluorescent semiconductor nanocrystals [ also known as quantum dots ( QDs ) ] were applied, which were nanometer - sized light - emitting particles and were emerging as a new class of fluorescent probes for cancer detection due to the unique optical and electronic properties.The technique of QDs immunofluorescence histochemistry ( QDs - IHC ) was used to detect the protein expression of EMMPRIN.P53 and MMP -2 in the human lung cancer microarray, and co - expression of EMMPRIN/P53 proteins was also simultaneously detected by douhle - laheling immunofluorescence.RESULTS : Compared with non - cancer ous lung tissues, the positive rates of EMMPRIN, P53 and MMP -2 proteins in the lung cancer tissues were 70.00％ ,77.14％ and 72.86％ , respectively, and the differences were all significant ( P ＜ 0.05 ).The positive rates of EMMPRIN ,P53 and MMP -2 proteins were all significantly related with tumor staging( TNM stage ) and lymph node metastasis ( P ＜0.05 ).The positive correlation between EMMPRIN expression and protein levels of MMP - 2 and P53 was observed ( P ＜0.01 ).CONCLUSION : The protein expression of EMMPRIN, P53 and MMP -2 is correlated with the development of lung cancer.Malignant progression of lung cancer promoted by EMMPRIN may be closely related with the expression of MMP - 2and P53.