六味地黄丸含药血清对HK-2细胞TGF-β/Smad信号通路的影响

摘要

目的:观察六味地黄丸含药血清对HK-2细胞增殖及其Smad通路信号影响.方法:MTT法检测大鼠5%、10%、20%空白血清与5%、10%、20%含药血清对HK-2细胞增殖的影响,并采用蛋白印迹法检测10%含药血清对HK-2细胞Smad通路信号分子蛋白表达的影响.结果:六味地黄丸含药血清可以促进HK-2细胞增殖;在HK-2细胞中,TGF-β1可明显促进Smad2蛋白的磷酸化,而10%六味地黄丸含药血清具有明显的抑制效应.10%六味地黄丸含药血清亦可促进SnoN蛋白表达,明显高于对照组和空白大鼠血清组.结论:六味地黄丸含药血清能够抑制TGF-β1诱导的HK-2细胞Smad2蛋白的磷酸化,促进TGF-β/Smad通路的负性调控因子SnoN蛋白的表达.%AIM : To investigate the effect of the drug - containing serum of Liuwei Dihuang pills on the TGF β1/Smad signaling pathway in HK - 2 cells.METHODS : The proliferation of HK - 2 cell was detected by MTT method.Western blotting analysis was used to investigate the effect of the drug - containing serum of Liuwei Dihuang pills on the protein expression of the molecules of Smad signal transduction pathway.RESULTS : The drug - containing serum of Liuwei Dihuang pills promoted the proliferation of HK - 2 cells.The level of Smad2 phosphorylation in HK - 2 cells treated with 10％ drug - containing serum of Liuwei Dihuang pills was significantly lower than that in the cells treated with TGF - β1 .Furthermore, SnoN, a negative factor in Smad signaling pathway, was up - regulated in HK - 2 cells treated with 10％drug - containing serum.CONCLUSION : Drug - containing serum of Liuwei Dihuang pills inhibits TGF - β1/Smad signaling pathway, including reducing Smad2 phosphorylation and promoting SnoN protein expression.