AIM; To invesligale the balance of Treg/Thl7 in synovium of collagen - induced arlhrilis (CIA) and the impacl of Lumor necrosis faclor ct(TNF - α) blockage therapy. METHODS; RaL CIA model was established by bovine II collagen injeclion. The palhological score was evaluated by HE slaining and Loluidine blue slaining. The TNF - α level in plasma was measured by ELISA. The expression of Treg/Thl7 in synovium was delecled by double slaining immu-nofluorescence. RESULTS; The plasma level of TNF - α in CIA group was significantly higher than that in conlrol group and TNFR - Fc Irealmenl group ( P < 0. 01) , whereas no significant difference was found between TNFR - Fc treatment group and control group ( P > 0. 05 ) . No significant difference between CIA group and control group in the ratio of CD4 + Foxp3 + Treg cells/CD4 + cells in synovium (23. 12% ±4.93% vs 24. 66% ±5.82%, P>0.05) was observed, whereas the ratio in TNFR - Fc treatment group was significantly increased( 33. 07% ± 5. 14% ) . The ratio of CD4 + ROR-yt* Thl7 cells/CD4 + cells in CIA group was significantly higher than that in control group and TNFR - Fc treatment group (9. 74% ±2.23% vs 1.00% ±0.59%, 5.63% ±1.76%, P<0.01). CONCLUSION; Differentiation disturbance of Treg/Thl7 exists in the synovium of CIA rats. TNFR - Fc may restore the balance of Treg/Thl7 by inhibiting Thl7 cell differentiation and inducing the production or accumulation of Treg.%目的:探讨胶原诱导性关节炎(CIA)关节滑膜Treg/Th17平衡状态以及TNF-α拮抗剂TNFR-Fc对Treg/Th17的影响.方法:建立CIA模型,观察踝关节组织病理变化,ELISA检测血浆肿瘤坏死因子α(TNF-α),免疫荧光双标法检测关节滑膜Treg/Th17的表达.结果:CIA组TNF-α较正常对照组及TNFR-Fc治疗组显著升高(P<0.01),TNFR-Fc治疗组与正常对照组间差异无统计学意义(P>0.05);CIA组滑膜组织的CD4+Foxp3+Treg/CD4+细胞比例与正常对照组比较无明显差异(23.12%±4.93% vs 24.66%±5.82%,P>0.05),而TNFR-Fc治疗组则升高明显(33.07%±5.14%);CIA组CD4+RORγt+Th17/CD4+细胞比例显著增高(9.74%±2.23% vs 正常对照组1.00%±0.59%,TNFR-Fc治疗组5.63%±1.76%,P<0.01).结论:CIA大鼠关节滑膜存在Treg/Th17分化失衡;TNFR-Fc可能通过抑制关节滑膜Th17细胞分化,诱导Treg细胞生成/聚集,使得病变关节的Treg/Th17平衡得以恢复.
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