儿童变应性鼻炎严重度与TGFB1基因-509 C/T多态性的关系

摘要

Objective To investigate the association between the promoter polymorphism -509C/T of the transforming growth factor-pi gene (TGFB1) and the disease severity of allergic rhinitis (AR) in childhood. Methods A total of 96 Chinese patients with persistent AR aged 3-17 (9. 4 ±3. 8) years old were enrolled in the study. Among these patients 53. 1% were mild cases (n = 51 ) and 46.9% were moerate-to-severe cases (n = 45). Genotyping was performed on peripheral blood genomic DNA by using PCR-RFLP. Serum levels of TGF-β1 was measured by ELISA, and serum total IgE, specific IgE and eosinophil cationic protein (ECP) levels were determined using an ImmunoCAPlOOE system. Statistical analysis was conducted with SPSS11.0 software. Results Significant differences were found in genotype frequencies for the TGFB1 - 509C/T polymorphism between mild and moderate-to-severe AR patients (X2 = 8. 361 , P =0. 015 ). Children with persistent AR bearing the TT genotype of the - 509C/T polymorphism had significantly increased risk for moderate-to-severe AR (Fisher's exact test, P=0.007) compared to children with the CC/CT genotypes. There was no significant association between the - 509C/Tpolymorphism and serum TGF-β1 levels (F=0. 389,P=0. 679) ; however, serum total IgE (F = 4.210,P = 0.018) and ECP ( H = 6. 297, P = 0. 043) levels were found to be significantly associated with the polymorphism. Conclusion The results suggest that the TGFB1 gene polymorphism - 509C/T may play a potential role in the severity of persistent AR in childhood.%目的 探讨儿童变应性鼻炎(allergic rhinitis,AR)严重度与转化生长因子β1(transforming growth factor β1,TGF-β1)基因(TGFB1)启动子区-509C/T多态性的相关性.方法 儿童持续性AR患者96例,年龄3～17岁,平均((x)±s)年龄(9.4±3.8)岁;其中轻度51例(53.1%),中-重度45例(46.9%).应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测患儿外周血中TGFB1基因-509C/T多态性的基因型,双抗体夹心ELISA法测定血清TGF-β1水平,ImmunoCAP100E变应原体外检测系统测定血清总IgE、特异性IgE和嗜酸粒细胞阳离子蛋白(eosinophil cationic protein,ECP)水平.使用SPSS11.0统计学软件对实验结果进行分析.结果 TGFB1基因-509C/T多态性在轻度AR组和中-重度AR组中的基闪型分布差异有统计学意义(x2=8.361,P=0.015),TT基因型与CC/CT基因型相比,在中-重度AR组中的频率显著高于轻度AR组(Fisher's精确概率法,P=0.007).该多态性位点的各基凶型之间,患者血清TGF-β1水平的差异无统计学意义(F=0.389,P=0.679),但血清总IgE水平(F=4.210,P=0.018)和ECP水平(H=6.297,P=0.043)的差异均有统计学意义.结论 TGFB1基因-509C/T多态件对儿童持续性AR的严重度可能有一定影响.