膝关节骨性关节炎患者尿液中C2C水平检测

摘要

Objective To observe the relationship between the urine C2C level and the severity of knee osteoarthritis (KOA). Methods Ninety patients with KOA were selected as KOA group , and 20 healthy volunteers were selected as control group .Five ml morning urine of each subject was collected and stored at -80℃.The anterior-posterior and lateral position of X-ray detection of knee joint was performed .The X-ray images were classified according to the Kellgren-Lawrence radiographic grading criteria .All the subjects were divided into 5 groups:control group, group I, group II, group III, and group IV, with no significant difference of age and sex.The urine C2C level was detected using enzyme-linked immunosorbent assay (ELISA).Results The urine C2C level in KOA group (218.341 ±22.270 pg/mL) was significantly higher than that in control group (261.235 ±39.944 pg/mL) ( P<0.01).The urine C2C level in group IV, group III, group II, group I, and control group was 311.872 ±18.759 pg/mL, 273.753 ±18.595 pg/mL, 256.251 ±23.379 pg/mL, 219.009 ±13.431 pg/mL, and 218.341 ±22.270 pg/mL, respectively, and the difference was significant (P=0.000).No significant difference of the urine C2C level between group I and control group was observed (P>0.05).Conclusion The urine C2C level in patients with KOA was higher than that in normal subjects .And the elevation of urine C2C level is parallel to the severity of KOA , showing a high-positive correlation.%目的：观察并探讨尿液中Ⅱ型胶原羧基末端3/4片段（ C2C）水平与膝关节骨性关节炎（ KOA）患者病变程度的关系。方法收集90例膝关节骨性关节炎患者及20例非膝关节骨性关节炎患者清晨尿液标本5 mL及膝关节正侧位影像学资料，尿液标本置-80℃冰箱保存，影像学资料根据Kellgren-Lawrence 放射学分级标准对膝关节骨性关节炎患者的影像学表现进行分级，实验分为五组，对照组，I级组，II级组，III级组及IV级组。各组间年龄及性别构成比较没有显著性差异，无统计学意义。尿液标本收齐后采用酶联免疫吸附试验（ELISA）检测尿液中C2C水平。结果与对照组（261.235±39.944 pg/mL）相比，膝关节骨性关节炎组C2C水平（218.341±22.270 pg/mL）明显升高，差异有统计学意义（ P＜0.01）。各组C2C水平比较：IV组（311.872±18.759 pg/mL）＞III组（273.753±18.595 pg/mL）＞II组（256.251±23.379 pg/mL）＞I组（219.009±13.431 pg/mL）或正常组（218.341±22.270 pg/mL）（P＝0.000）。 I级膝关节骨性关节炎组患者尿液C2C与对照组水平相差无统计学意义（ P＞0.05）。结论骨性关节炎患者C2C水平较正常对照组高，C2C升高水平与膝关节骨性关节炎病变程度平行，且二者呈高度正相关。