胃癌患者血清、尿液骨桥蛋白水平检测的临床分析

摘要

目的 检测胃癌患者血清和尿液中骨桥蛋白((ostoepontin,OPN)含量,探讨其在胃癌辅助诊断,监测病情变化,判断预后中的作用.方法 应用酶联免疫吸附试验检测60例胃癌患者,42例胃溃疡患者,33例健康体检者血清和尿液中OPN含量.结果 胃癌组患者血清、尿液中OPN(104.4±36.3)μg/L,(230.0±46.7)μg/L显著高于溃疡组(47.8±29.6)μg/L,(98.9±40.2)μg/L及体检组(36.5±24.8)μg/L,(86.0±35.2)μg/L(P<0.01),尿液中的含量明显高于血清中含量(P <0.01);OPN在血清、尿液中含量与胃癌的临床分期、肿瘤大小、琳巴结转移及远处转移有关;血清和尿液中的OPN值呈正相关(r=0.92,P<0.01).结论血清和尿液中的OPN水平可反映胃癌患者机体肿瘤负荷状态,可作为诊断、监测病情变化、判断预后有价值的指标.%Objective This research investigated the expression level of ostoepontin(OPN) in serum and urine of gastric cancer patients .also approached the effects of OPN in diagnosis and monitoring of the course and prognosis of gastric cancer. Methods The concentration of OPN in 60 serum and urine samples of gastric cancer patients.42 serum and urine samples of gastric ulcer patients and 33 serum and urine samples of healthy persons were measured by enzyme-linked immunosorbent assay. Results The expression levels of OPN in serum and urine of gastric cancer patients (104. 4±36. 3) μg/L,(230. 0±46. 7) μg/L were significantly higher than those in serum and urine of gastric ulcer patients (47.8±29.6) μg/L,(98. 9±40. 2) μg/L and healthy examination persons (36.5±24. 8) μg/L,(86.0± 35. 2) μg/L( P <0. 01). The expression levels of OPN in serum and urine of gastric cancer patients were higher along with PTNM stage,the size of the tumor, the metastasis of lymph node and distant metastasis. The concentrations of both serum and urinary OPN had positive correlation ( r =0. 92, P <0.01). Conclusion The concentrations of both serum and urinary OPN can reflex the tumor load of gastric cancer, the assay of OPN in serum and urine of gastric cancer patients is helpful in diagnosis,predicting the risk for metastasis and prognosis in gastric cancer.