骨连蛋白在年龄相关性白内障晶状体上皮细胞中的表达

摘要

目的 探讨骨连蛋白在皮质性及核性白内障晶状体上皮细胞中的表达.方法 实验研究.2010年5至7月,收集33例(45只眼)单纯性皮质性及核性白内障患者手术中取下的晶状体前囊膜,分为皮质组和核性组.免疫组织化学法观察骨连蛋白在晶状体上皮细胞中的表达,并进行图像分析;TUNEL法测定晶状体上皮细胞的凋亡情况.LSD-t检验分析两种类型白内障晶状体上皮细胞中骨连蛋白的表达及细胞凋亡指数的差异.结果 用于免疫组织化学检测的囊膜共20例(皮质性8例,核性12例),用于TUNEL法检测的囊膜共25例(皮质性9例,核性16例).在囊膜的周边部与中央部,骨连蛋白表达在核性白内障组均多于皮质性白内障组(周边部:t =2.827,P＜0.05;中央部t=2.264,P＜0.05),而细胞凋亡指数在皮质性白内障组(3.96±0.83,5.43±0.92)多于核性白内障组(0.89±0.74,1.07±0.53)(周边部:t=3.473,P＜0.05;中央部t=2.771,P＜0.05),差异均有统计学意义.在核性组与皮质组,骨连蛋白在囊膜周边部表达明显高于中央部(核性组:t=2.364,P＜0.05;皮质组:t=2.723,P＜0.05),细胞凋亡指数在周边部明显小于中央部,差异均有统计学意义(核性组:t=2.771,P＜0.05:皮质组:t=2.473,P＜0.05).结论 骨连蛋白表达在两种类型年龄相关性白内障晶状体上皮细胞中表达存在差异,并且在晶状体囊膜上存在空间差异性,此差异与晶状体上皮细胞凋亡有关.%Objective To study the expression of osteonectin ( also called secreted protein acidic and rich in cysteine,SPARC) in the lens epithelium cells in cortex cataract and nucleus cataract.Method This is an experimental research.Forty-five capsuiotomy specimens were obtained from 33 patients suffered form pure cortex orand nucleus cataracts and classified as nucleus group and cortex group,between May to July of 2010.Immunohistochemistry was used to detect the SPARC,which was analyzed by gray value.TUNEL was used to examine the apoptosis of lens epithelium cell.The difference of SPARC expression and apoptosis rate between these two groups was analyzed by LSD-t.Results Twenty specimens were studied with immunohistochemistry ( C/N =8/12 ),and 25 specimens studied by TUNEL test ( C/N =9/16 ).Expression of SPARC in the cortex group was significantly less than that in the nucleus group ( peripheral region:t =2.827,P ＜ 0.05 ; central region:t =2.264,P ＜ 0.05 ).Expression of SPARC in the central region was significantly less than that in the peripheral region (peripheral region:t =3.473,P ＜ 0.05; central region:t =2.771,P ＜ 0.05 ).Apoptosis rate in the nucleus group was significantly lower than the cortex group( nucleus group:t =2.364,P ＜ 0.05 ; cortex group:t =2.723,P ＜ 0.05 ).Apoptosis rate in peripheral region was significantly less than that in central region( nucleus group:t =2.771,P ＜0.05 ;cortex group:t =2.473,P ＜ 0.05 ).Conclusions The expression of SPARC is different between nucleus cataract and cortex cataract and also different between central and peripheral regions.The expression of SPARC is related with apoptosis of lens capsule epithelium cells.