isolated fibronectin binding protein (fnb), altered from staphylococcal aureus (s. aureus); fnb isolated, altered; immunogenic composition; method for immunizing a vertebrate against s. aureus; expression vector; recombinant host cell; method for producing an altered fnb; method for inducing an immune response in a vertebrate; isolated, altered fibronectin binding protein (fnb) from staphylococcal aureus (s. aureus); and isolated nucleic acid molecule encoding an altered fnb of s. aureus

摘要

ISOLATED, ALTERED STAPHYLOCOCCAL AUREUS (S. AUREUS) BINDING PROTEIN; FNB ISOLATED, CHANGED; IMMUNOGENIC COMPOSITION; METHOD FOR IMMUNIZING A VERTEBRATE AGAINST S. AUREUS; EXPRESSION VECTOR; RECOMBINANT HOST CELL; METHOD FOR PRODUCING AN ALTERED FNB; METHOD FOR INDUCING AN IMMUNOLOGICAL RESPONSE IN A VERTEBRATE; ISOLATED, CHANGED STAPHYLQCOCCAL AUREUS 3. AUREUS FIBRONECTIN (FNB) BINDING PROTEIN; AND NUCLEIC ACID ISOLATED MOLECULE ENCODING A CHANGE OF 3. AUREUS FNB An isolated, altered 3. aureus protein (Fnb) is described that has at least one mutation in an amino acid selected from residues corresponding to Gln103, Gln105, Lys157, Lys503, Lys620, Lys702, Lys762, Gln783 and Gln830 of the S. aureus strain ATCC49S525. Substitution of these reactive residues within the fibronectin binding protein makes this protein less capable than wild-type covalently cross-linked Fnb with fibronectin and fibrin. Altered fibronectin binding protein effectively interferes with S. aureus adhesion to fibronectin and fibrin and, consequently, an immunogenic composition comprising such altered Fnb exhibits improved immunogenic properties and is safer to use.