白藜芦醇对胶质瘤U87细胞迁移和侵袭能力的影响

摘要

Objective To evaluate the effects of resveratrol (Rev) on migration and invasion of glioma cell line U87 cells and its mechanism. Methods Viability of glioma U87 cells treated with Rev in variable concentrations for 24 h and 48 h was detected by methyl thiazolyl tetrazolium (MTT) assay. The migration and invasion were evaluated by in vitro scratch assay and Boyden chamber assay respectively; matrix-metalloproteinase (MMP) activity was determined by gelatine zymography assay. Results MTT assay showed Rev inhibited the proliferation of glioma U87 cells in a dose- and time-dependent manner (P<0.05); compared with control group, the migration and invasion of U87 cells treated with Rev were decreased obviously, accompanied by the inhibition of MMP-2 activity. Conclusion Rev can inhibit the migration and invasion ability of glioma U87 cells. The inhibitive effects may be associated with the decreased MMP-2 activity.%目的 探讨白藜芦醇对胶质瘤U87细胞迁移和侵袭能力的影响和作用机制.方法 采用四唑盐(MTT)比色实验检测胶质瘤U87细胞经不同浓度白藜芦醇作用24 h、48 h、72 h后生存率的变化；细胞划痕试验和transwell侵袭试验检测白藜芦醇对胶质瘤U87细胞迁移和侵袭能力的影响；明胶酶谱法分析白藜芦醇对细胞基质金属蛋白酶(MMP)活性的改变.结果 MTT法显示一定浓度的白藜芦醇可抑制U87细胞生长,并随着浓度的增加和作用时间的延长而明显增强(P＜0.05)；与对照组比较,经白藜芦醇作用后U87细胞迁移和侵袭能力明显降低,MMP-2分泌减少.结论 白藜芦醇可有效抑制胶质瘤U87细胞迁移和侵袭能力,其机制可能与白藜芦醇降低细胞MMP-2活性相关.