血管紧张素原、血管紧张素转换酶基因多态性 与脑卒中相关性研究

摘要

Objective　To investigate the association of the M235T polymorphism of the angiotensinogen (AGT) gene,the insertion (I)/deletion (D) polymorphism of the angiotensin converting enzyme gene and cerebral strokes in Chinese.　Methods　The PCR-RFLR was used to test the M235T polymorphism of AGT and the D/I polymorphism of ACE genes of 100 cerebral stroke patients (72 patients with cerebral infarction,28 patients with cerebral hemorrhage) and 100 normal people. Results　Frequency of the homozygous insertion genotype and I allele of ACE increase（x2=4.17,P=0.041）,while the 235TT genotype and T allele of AGT gene also increased significantly（x2=26.79,P0.001）.　Conclusion　Contemporary test for the polymorphism of AGT and ACE genes is a method to evaluate the risk factors for acute cerebrovascular diseases at molecular level,which is very important for selecting high-risk population of cerebral strokes and taking measures for their protection and therapy.%目的　探讨血管紧张素原基因（AGT）的M235T基因多态性、血管紧张素转换酶（ACE）基因的插入（I）/缺失（D）多态性与中国汉族人脑卒中发病的相关性。方法　采用PCR法和PCR-RFLR法检测100例脑卒中患者(脑梗死患者72例,脑出血患者28例)以及100例正常人ACE（I/D）和AGT（M235T）的基因多态性。结果　在脑卒中患者中ACE的纯合插入（I）基因型和I等位基因频率增加 (x2=4.17,P=0.041），AGT基因的235TT基因型和T等位基因频率明显增加（x2=26.79,P结论　同期监测AGT及ACE基因多态性是一项对急性脑血管病风险因素分子水平的检测方法，这对筛选脑卒中高危人群并早期采取干预对策，防治脑卒中有一定意义。