RKIP基因表达上调对宫颈癌细胞生物学行为影响

摘要

目的：分析Raf激酶抑制蛋白（RKIP）基因表达上调对宫颈癌细胞生物学行为的影响。方法：将含正义（ss）RKIP cDAN真核表达载体传入宫颈癌细胞，使用Westernblot法检测传染前后细胞RKIP基因表达及其上调的稳定转染细胞系（ssRKIP），观察RKIP基因表达上调对宫颈癌细胞生物学行为的影响。结果：ssRKIP细胞、空载体pcDNA3.1（+）及为转染细胞的相对表达含量分别为2.13、1.12和1.08，转然后ssRKIP细胞的RKIP蛋白表达水平上条明显，pcDNA3.0（+）细胞的RKIP蛋白表达水平变化不明显。ssRKIP细胞的克隆形成分数、穿膜细胞数显著低于PcDNA3.1（+）转染组和未转染组，差异有统学意义（P＜0.05），PcDNA3.1（+）转染组和未转染组的克隆形成指数和穿膜细胞数差异无统计学意义（P＞0.05）。结论：RKIP基因可能属于抑癌基因，RKIP表达上调对肿瘤细胞增殖和侵袭能力具有抑制效果，可作为治疗宫颈癌的新靶点。%Objective: To analyze the effect of Raf kinase inhibitor protein(RKIP)gene expression on the biological behavior of cervical cancer cell line.Methods:With justice(SS)RKIP cDAN eukaryotic expression vector was introduced into cervical cancer cells.Using Western blot were used to detect the infection before and after cell RKIP gene expression and upregulation of stably transfected cell lines ssRKIP,observation of RKIP gene expression hike impact on biological behavior of cervical cancer cell line.Results:ssRKIP cells and empty vector pcDNA3.1(+)and transfected cells and the relative expression levels were 2.13,1.12 and 1.08,and RKIP protein ssRKIP cell expression level obviously,pcdna3.0(+)cells of RKIP protein expression levels did not change significantly.The formation of ssRKIP cell clone fraction,transmembrane cell number was significantly lower than that of pcDNA3.1(+)transfection group and non transfection group,difference has statistical significance(P<0.05),pcDNA3.1(+)transfection group and non transfection group of clonal formation index and the transmembrane cell number difference of no statistical significance(P>0.05).Conclusion:RKIP gene may be a tumor suppressor gene,RKIP expression up regulation on tumor cell proliferation and invasion ability has inhibitory effect,can be used as a new target for the treatment of cervical cancer.