HLA-DP、DQ基因多态性与福建汉族人群乙型肝炎病毒感染及不同结局的关联研究

摘要

Objective To investigate the association of single nucleotide polymorphisms in the HLA-DP and DQ genes with the outcome of chronic hepatitis B virus infection.Methods Two hundred and four healthy subjects,255 clearance subjects,204 asymptomatic HBV carriers (AsC),136 chronic hepatitis B (CHB),68 liver cirrhosis (LC) and hepatocellular carcinoma (HCC) were enrolled.Genotypes of rs3077,rs9277535 and rs2647050 were determined by sequence specific primers-PCR (PCR-SSP).Results By using healthy subjects and clearance subjects as the control groups,rs3077 and rs9277535 were significantly associated with chronic HBV infection under additive and dominant models (P ＜ 0.05).Meanwhile,haplotypes GGA,AGA,AAA appeared to be protective factors against chronic HBV infection (P＜0.05).By using AsC as the control group,comparison with the CHB,LC and HCC groups showed no association of the 3 SNPs or haplotypes with the clinical outcome (P＞ 0.05).Conclusion HLA-DP gene polymorphisms are strongly associated with chronic HBV infection.The presence of A allele at rs3077 and rs9277535 of the HLA-DP gene may decreased the risk for chronic HBV infection.%目的 探讨HLA-DP、DQ基因单核苷酸多态性与乙型肝炎病毒(hepatitis B virus,HBV)感染及不同结局的相关性.方法 采用序列特异性引物PCR基因分型技术对健康人群(n=204)、自限性感染人群(n=255)、慢性HBV感染人群(无症状携带者204例、慢性乙型肝炎136例、肝硬化及肝细胞癌各68例)进行rs3077、rs9277535、rs2647050位点的多态性检测.结果 健康对照组、自限性感染组与慢性HBV感染组相比,rs3077、rs9277535位点在显性和加性模型下差异均有统计学意义(P＜0.05),而rs2647050位点差异无统计学意义(P＞0.05),单倍型GGA、AGA、AAA与HBV感染存在相关性(P＜0.05).以无症状携带者为对照组,分别与慢性乙型肝炎组、肝硬化组及肝细胞癌组相比,以上3个SNP位点与HBV感染结局差异均无统计关联(P＞0.05),也无单倍型与HBV感染结局存在关联(P＞0.05).结论 HLA-DP基因多态性与慢性HBV感染存在关联,rs3077A、rs9277535A是慢性HBV感染的保护因素.