Objective To study the prevalence,virulence and antimicrobial resistance of uropathogenic Escherichia coli gene types in the area.Methods Fifty Escherichia coli clinical isolated from outpatients and inpatients with urinary tract infection in the First Affiliated Hospital,College of Medicine,Zhejiang University from July 2011 to June 2012 were chosen.All isolates were assessed for phylogenetic group,sequence type,46 virulence genes and antimicrobial resistance.Virulence scores were compared between groups by Rank-sum test,while the rates of groups were compared by Chi-square test or Fisher exact test.Results The 55 isolates were divided into phylogenetic group A,B1,B2 and D,accounting for 9.1％,5.5％,43.6％ and 41.8％,respectively.The virulence scores (number of virulence genes) P50 were 6,7,14 and 9,respectively.The virulence scores of group A,B1 and D were significantly different from that of group B2 (P ＜0.001,0.005 and ＜0.001 respectively).ST73,ST95 and ST493 isolates (9,16.4％) belonging to group B2,exhibited virulence genes up 15 to 20.B2-ST131 was the most common isolate,accounting for 20.0％ of the overall isolates.The ST131 isolates exhibited higher antimicrobial resistance to 13 kinds of antimicrobials than non-ST131 isolates.The resistance rate to ciprofloxacin of the ST131 isolates was 90.9％ (10/11).Conclusions It is important to pay attention to high prevalence of some UPEC clonal groups with enhanced virulence and(or) antimicrobial resistance in the area.And larger range of surveillance should be emphasized.%目的 了解杭州地区尿道致病性大肠埃希菌(UPEC)基因型的构成以及毒力和耐药性.方法 收集浙江大学医学院附属第一医院2011年7月至2012年6月从尿路感染患者尿液中分离的55株UPEC,对其开展系统分群和多位点序列分型,筛查46种毒力基因,采用纸片扩散法开展药敏试验.毒力分数组间比较采用秩和检验,率的组间比较采用卡方检验或Fisher检验.结果 55株UPEC中A、B1、B2和D群分别占9.1％、5.5％、43.6％和41.8％,毒力分数(毒力基因个数)的第50百分位数P50分别为6、7、14和9,A、B1和D群的毒力分数分别与B2群的比较,均有显著性差异(P分别为＜0.001、0.005和＜0.001).B2群中的ST73、ST95和ST493菌株(共9株,16.4％)的毒力分数高达15 ～ 20.11株(20.0％)ST131均属于B2群,是最常见的克隆群.有13种药物的耐药率ST131菌株大于非ST131,ST131菌株对环丙沙星耐药率高达10/11.结论 杭州地区UPEC中存在强毒力和(或)高耐药性的克隆株,必须高度重视,避免广泛流行.
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