RNA 采集系统在血液分子诊断生物标志物检测中的应用

摘要

血液样本作为生物样本库的重要成员，含有丰富的生物分子可用于疾病诊断、判断疾病分期及预后等。血液样本较组织样本具有更容易获取、可连续采样及检测项目丰富等优势。然而，血液样本收集后需进行处理、分装并冻存于适合长期冰冻保存的容器中。否则，血液中 RNA 在常温运输及日常保存条件下容易发生降解，从而影响后续分子诊断生物标志物的检测、分析及研发。本文阐释了目前临床及科研工作中常规血液采集和保存方法对血液 RNA 降解的影响及常见的解决方案。（中华检验医学杂志，2016，39：559－561）%As one important member of the biological sample library , blood samples are rich in biological molecules, which can be used for disease diagnosis , prognosis of disease staging , and prognosis et al.compared with tissue samples, blood samples have some advantages , such as easier to access, sampling in a row and rich test items and so on.However, after blood samples were collected, which need to be processed, packed and frozen in ice for long-term preservation container.Otherwise RNA in the blood sample is unstable and easily degradation in normal temperature transportation and preservation condition , thus subsequently affecting the molecular diagnostic biomarkers ′detection, analysis, research and development.In this paper, This review summarizes the current clinical and research work of routine blood collection and preservation methods effecting on blood RNA degradation and solution for avoiding these degrading which can help testing changings of blood biomarker more accurately .