目的 观察沉默G250基因对肾癌786-0细胞凋亡的影响.方法 构建靶向G250的siRNA表达质粒pshRNA-G250并转染肾癌786-0细胞,TUNEL法和流式细胞仪检测G250基因沉默后肾癌786-0细胞(命名786-0/si-G250-b)的凋亡率,以转染空质粒的细胞(命名786-0/si-G250-0)为对照.结果 TUNEL法显示786-0/si-G250-b和786-0/si-G250-0细胞凋亡率分别为(21.537±3.552)%、(6.590±1.167)%,(t=6.925,P=0.002),流式细胞仪检测示细胞凋亡率分别为(33.033±2.914)%、(9.133±1.115)%,(t=13.266,P=0.000).结论 沉默G250基因的表达可诱导肾癌786-0细胞的明显凋亡.%Objective To investigate the effects of G250 gene silencing by siRNA on apoptosis of renal cell carcinoma 786-0 cell .Methods The eukaryotic expression vector named pshRNA-G250 of small interfering RNA (siRNA ) targeting G250 gene was constructed and transfected into renal cell carcinoma 786-0 cells .TUNEL and flow cytometry (FCM ) assay were performed to test the rate of apoptosis of 786-0 cells after G250 gene silencing (named 786-0 /srG25O-b) .and one that empty plasmid was transfected into (named 786-0/si-G250-0) as control group .Results TUNEL showed apoptosis rate of 786-0/si-G250-b) and 786-0/si-G250-0 cell was (21.537± 3.552)％ ,(6.590 ± 1.167)％ ,(t=6 .925 , ρ=0 .002) .PCM showed the rate of apoptosis was (33.033 ± 2.914)％,(9 .133±1.115)％ ,(t=13 .266 ,ρ=0 .000),respectively .Conclusion The inhibition to the expression of G250 gene can obviously induce the apoptosis of renal carcinoma 786-0 cells .
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