谷氨酰半胱氨酸连接酶活性在系统性红斑狼疮中的变化及意义

摘要

目的：探讨 SLE 人群和正常对照组外周血淋巴细胞中谷氨酰半胱氨酸连接酶（GCL）活性的差异。方法收集30例活动期的 SLE 病例与30名正常对照，利用密度梯度离心法提取外周血淋巴细胞，酶法测定外周血淋巴细胞中 GCL 的活性水平。同时分析合并和未合并狼疮肾炎的 SLE 组之间 GCL 活性水平的差异。结果与对照组（383．27±18．68 mmol/min/mg 蛋白浓度）相比较，SLE 组（266．10±13．31 mmol/min/mg 蛋白浓度）GCL 活性水平明显下降（P ＜0．0001）；与未合并狼疮肾炎（LN）的 SLE 组（303．25±18．47 mmol/min/mg 蛋白浓度）相比较，合并LN 的 SLE 患者 GCL 平均活性水平（229.64±17．82 mmol/min/mg 蛋白浓度）显著减低（P ＜0．009）。结论SLE 患者 GCL 活性异常，可能在系统性红斑狼疮组发病和病情进展中起一定作用。%Objective To investigate whether glutamate cysteine ligase (GCL)activity is abnormal in peripheral blood mononuclear cells (PBMC)of systemic lupus erythematosus (SLE)patients.Methods The clinical data of 30 systemic lupus erythematosus patients and 30 normal controls were enrolled.PBMC was separated by density gradient centrifugation using the Ficoll Paque system.GCL activity in PBMC was determined by a enzyme fluorescence assay. Results GCL activity was decreased (P <0.0001)in SLE patients (266.10±13.31 mmol/min/mg protein)compared with healthy controls (383.27 ± 18.68 mmol/min/mg protein).Moreover,GCL activity in SLE patients with LN (229.64±17.82 mmol/min/mg protein)was significantly decreased(P < 0.009)compared with those SLE patients without LN (303.25±18.47 mmol/min/mg protein).Conclusion Abnormal GCL activity was found in PBMC of SLE patients.GCL activity may play roles in the pathogenesis of SLE.