目的：探讨肝细胞生长因子（HGF）对血管紧张素II（AngII）介导的肾小管上皮细胞转分化（TEMT）的影响以及是否与信号转导和基因转录激活子（STAT3）信号转导通路有关。方法体外培养人近曲小管上皮细胞并分为4组，即对照组，AngII（AngII：10－6 mol／L）组，HGF（HGF ：8μg）组，AngII（AngII：10－6 mol／L）＋ HGF（HGF ：8μg）组，用RT‐PCR的方法检测4组中α‐平滑肌肌动蛋白（α‐SMA）及β‐actin的 mRNA 表达，用 Western方法检测4组中 P‐STAT3及β‐actin的蛋白表达。结果与对照组相比，HGF组α‐SMA mRNA表达减少，P‐STAT3蛋白表达下降， AngII组与之相反；与 AngII 组比较，AngII＋ HGF组α‐SMA mRNA表达减少，P‐STAT3蛋白表达下降。结论HGF可抑制AngII介导的肾小管上皮细胞转分化，且此作用可能通过对STAT3信号转导通路的抑制而达到的。%Objective To inquire the effects of hepatocyte growth factor (HGF) on tubulapithelial‐myofibroblast transdifferentiation (TEMT) that Angiotensin II (Ang II) took part in ,and the associated study about STAT3 signa‐ling pathway .Methods HK‐2 cells were cultured in RPMI‐1640 medium containin ,and the cells were divided into four groups :control ,AngII(AngII:10-6mol/L) group ,HGF(HGF :8 μg)group ,AngII(AngII:10-6mol/L)+ HGF(HGF :8μg)group .α‐smooth muscle actin (α‐SMA) ,andβ‐actin mRNA expressions were detected by RT‐PCR ,P‐STAT3 andβ‐actin protein expressions were detected by Western .Results α‐SMA mRNA expression was reduced and STAT3 pro‐tein expression were reduced in HGF group compared to control .But α‐SMA mRNA expression ,STAT3 protein ex‐pression were elevated in AngII group .α‐SMA mRNA ,STAT3 protein expression were all released in AngII+ HGF group compared to AngII group .Conclusion HGF can restrain TEMT that Ang II took part in ,and this effect maybe realized by inhibiting STAT3 signaling pathway .
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